Functional analysis of late-budding domain activity associated with the PSAP motif within the vesicular stomatitis virus M protein

Takashi Irie, Jillian M. Licata, Himangi R. Jayakar, Michael Whitt, Peter Bell, Ronald N. Harty

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A PPPY motif within the M protein of vesicular stomatitis virus (VSV) functions as a late-budding domain (L-domain); however, L-domain activity has yet to be associated with a downstream PSAP motif VSV recombinants with mutations in the PPPY and/or PSAP motif were recovered by reverse genetics and examined for growth kinetics, plaque size, and budding efficiency by electron microscopy. Results indicate that unlike the PPPY motif, the PSAP motif alone does not possess L-domain activity. Finally, the insertion of the human immunodeficiency virus type 1 p6 L-domain and flanking sequences into the PSAP region of M protein rescued budding of a PPPY mutant of VSV to wild-type levels.

Original languageEnglish (US)
Pages (from-to)7823-7827
Number of pages5
JournalJournal of Virology
Volume78
Issue number14
DOIs
StatePublished - Jul 1 2004

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Vesiculovirus
Vesicular Stomatitis
Viruses
Reverse Genetics
HIV-1
Electron Microscopy
proteins
Human immunodeficiency virus 1
Mutation
electron microscopy
Growth
mutation
kinetics
mutants
Proteins
Vesicular stomatitis virus M protein

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Functional analysis of late-budding domain activity associated with the PSAP motif within the vesicular stomatitis virus M protein. / Irie, Takashi; Licata, Jillian M.; Jayakar, Himangi R.; Whitt, Michael; Bell, Peter; Harty, Ronald N.

In: Journal of Virology, Vol. 78, No. 14, 01.07.2004, p. 7823-7827.

Research output: Contribution to journalArticle

Irie, Takashi ; Licata, Jillian M. ; Jayakar, Himangi R. ; Whitt, Michael ; Bell, Peter ; Harty, Ronald N. / Functional analysis of late-budding domain activity associated with the PSAP motif within the vesicular stomatitis virus M protein. In: Journal of Virology. 2004 ; Vol. 78, No. 14. pp. 7823-7827.
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AU - Bell, Peter

AU - Harty, Ronald N.

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