Functional expression of rat renal cortex taurine transporter in Xenopus laevis oocytes

Adaptive regulation by dietary manipulation

Xiaobin Han, Andrea M. Budreau, Russell W. Chesney

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Renal brush border taurine transport adapts to changes in the dietary intake of sulfur amino acids with increased rates after dietary restriction and reduced transport after dietary surplus. The Xenopus laevis oocyte expression system was used to define the renal adaptive response to dietary manipulation. Injection of poly(A)+ RNA isolated from rat kidney cortex resulted in a time and dose dependent increase in NaCl-taurine cotransport in oocytes. The K(m) of the expressed taurine transporter was 22.5 μM. In oocytes, injection of 40 ng of poly(A)+ RNA from kidneys of low taurine diet (LTD)-fed rats elicited 2-fold the taurine uptake of normal taurine diet (NTD)-fed rats and >3-fold the uptake of high taurine diet (HTD)-fed rats. Northern blots of rat kidneys using a riboprobe derived from an rB16a (rat brain taurine transporter) subclone revealed 6.2- and 2.4-kb transcripts, the abundance of which were increased or decreased in LTD- or HTD-fed rats, respectively, as compared with NTD-fed rats. A ~70-kD protein was detected by Western blot using an antibody derived front a synthetic peptide corresponding to a conserved intracellular segment of rB16a. The abundance of the ~70-kD protein was increased or decreased in LTD- or HTD-fed rats, respectively, as compared with NTD-fed rats. In conclusion, expression of the rat renal taurine transporter is regulated by dietary taurine at the level of mRNA accumulation and protein synthesis.

Original languageEnglish (US)
Pages (from-to)624-631
Number of pages8
JournalPediatric Research
Volume41
Issue number5
DOIs
StatePublished - Jan 1 1997

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Taurine
Xenopus laevis
Oocytes
Kidney
Diet
taurine transporter
Messenger RNA
Sulfur Amino Acids
Kidney Cortex
Injections
Proteins
Microvilli
Northern Blotting
Western Blotting

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health

Cite this

Functional expression of rat renal cortex taurine transporter in Xenopus laevis oocytes : Adaptive regulation by dietary manipulation. / Han, Xiaobin; Budreau, Andrea M.; Chesney, Russell W.

In: Pediatric Research, Vol. 41, No. 5, 01.01.1997, p. 624-631.

Research output: Contribution to journalArticle

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