Functional Interaction of Calcium-/Calmodulin-dependent Protein Kinase II and Cytosolic Phospholipase A2

Mubarack M. Muthalif, Ying Hefner, Stéphane Canaan, Jason Harper, Huilin Zhou, Jean Hugues Parmentier, Ruedi Aebersold, Michael H. Gelb, Kafait Malik

Research output: Contribution to journalArticle

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Abstract

Calcium-/calmodulin-dependent protein kinase II (CaM kinase II), a decoder of Ca2+ signals, and cytosolic phospholipase A2 (cPLA 2), an enzyme involved in arachidonate release, are involved in many physiological and pathophysiological processes. Activation of CaM kinase II in norepinephrine-stimulated vascular smooth muscle cells leads to activation of cPLA2 and arachidonic acid release. Surface plasmon resonance, mass spectrometry, and kinetic studies show that CaM kinase II binds to cPLA 2 resulting in cPLA2 phosphorylation on Ser-515 and an increase in its enzymatic activity. Phosphopeptide mapping studies with cPLA2 from norepinephrine-stimulated smooth muscle cells indicates that phosphorylation of cPLA2 on Ser-515, but not on Ser-505 or Ser-727, occurs in vivo. This novel signaling pathway for arachidonate release is shown to be cPLA2-dependent by use of a recently described and highly selective inhibitor of this enzyme.

Original languageEnglish (US)
Pages (from-to)39653-39660
Number of pages8
JournalJournal of Biological Chemistry
Volume276
Issue number43
DOIs
StatePublished - Oct 26 2001

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Cytosolic Phospholipases A2
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Calcium-Calmodulin-Dependent Protein Kinases
Phosphorylation
Muscle
Norepinephrine
Chemical activation
Cells
Smooth Muscle Myocytes
Phosphopeptides
Surface plasmon resonance
Enzyme Inhibitors
Physiological Phenomena
Arachidonic Acid
Mass spectrometry
Surface Plasmon Resonance
Vascular Smooth Muscle
Mass Spectrometry
Kinetics
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Functional Interaction of Calcium-/Calmodulin-dependent Protein Kinase II and Cytosolic Phospholipase A2. / Muthalif, Mubarack M.; Hefner, Ying; Canaan, Stéphane; Harper, Jason; Zhou, Huilin; Parmentier, Jean Hugues; Aebersold, Ruedi; Gelb, Michael H.; Malik, Kafait.

In: Journal of Biological Chemistry, Vol. 276, No. 43, 26.10.2001, p. 39653-39660.

Research output: Contribution to journalArticle

Muthalif, MM, Hefner, Y, Canaan, S, Harper, J, Zhou, H, Parmentier, JH, Aebersold, R, Gelb, MH & Malik, K 2001, 'Functional Interaction of Calcium-/Calmodulin-dependent Protein Kinase II and Cytosolic Phospholipase A2', Journal of Biological Chemistry, vol. 276, no. 43, pp. 39653-39660. https://doi.org/10.1074/jbc.M103136200
Muthalif, Mubarack M. ; Hefner, Ying ; Canaan, Stéphane ; Harper, Jason ; Zhou, Huilin ; Parmentier, Jean Hugues ; Aebersold, Ruedi ; Gelb, Michael H. ; Malik, Kafait. / Functional Interaction of Calcium-/Calmodulin-dependent Protein Kinase II and Cytosolic Phospholipase A2. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 43. pp. 39653-39660.
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