Further studies on metabolism of phosphatidic acid of isolated E. coli membrane vesicles

Edwin Thomas, Herbert Weissbach, H. R. Kaback

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Membrane-bound [32P]-phosphatidic acid, labeled by incubation of E. coli membrane vesicles with [γ-32P]-ATP, exchanges 32P with ATP and other nucleoside triphosphates, and phosphorylates ADP by a process independent of oxidative phosphorylation. In the presence of CTP, label from [32P]-phosphatidic acid appears principally in phosphatidyl ethanolamine and phosphatidyl glycerol, apparently by exchange of the phosphatidyl moiety of [32P]-CDP-diglyceride with membrane-bound phosphatidyl serine and phosphatidyl glycerol phosphate. The net conversion of phosphatidic acid to phosphatidyl ethanolamine and phosphatidyl glycerol is measured by the incorporation of radioactive serine and α-glycerol-P into the membrane phospholipids. Their incorporation requires CTP and Mn2+, and is stimulated by ATP and monovalent cations. The rate of incorporation of serine is not dependent on the transport of serine into the intravesicular space. When hydroxylamine is used to prevent decarboxylation of newly synthesized phosphatidyl serine, the serine moiety can exchange with exogenous serine.

Original languageEnglish (US)
Pages (from-to)797-806
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume150
Issue number2
DOIs
StatePublished - Jan 1 1972
Externally publishedYes

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Phosphatidic Acids
Metabolism
Serine
Escherichia coli
Phosphatidylglycerols
Membranes
Cytidine Triphosphate
Ethanolamine
Adenosine Triphosphate
Phosphatidylserines
Cytidine Diphosphate Diglycerides
Monovalent Cations
Hydroxylamine
Decarboxylation
Oxidative Phosphorylation
Nucleosides
Glycerol
Adenosine Diphosphate
Labels
Phospholipids

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Further studies on metabolism of phosphatidic acid of isolated E. coli membrane vesicles. / Thomas, Edwin; Weissbach, Herbert; Kaback, H. R.

In: Archives of Biochemistry and Biophysics, Vol. 150, No. 2, 01.01.1972, p. 797-806.

Research output: Contribution to journalArticle

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AB - Membrane-bound [32P]-phosphatidic acid, labeled by incubation of E. coli membrane vesicles with [γ-32P]-ATP, exchanges 32P with ATP and other nucleoside triphosphates, and phosphorylates ADP by a process independent of oxidative phosphorylation. In the presence of CTP, label from [32P]-phosphatidic acid appears principally in phosphatidyl ethanolamine and phosphatidyl glycerol, apparently by exchange of the phosphatidyl moiety of [32P]-CDP-diglyceride with membrane-bound phosphatidyl serine and phosphatidyl glycerol phosphate. The net conversion of phosphatidic acid to phosphatidyl ethanolamine and phosphatidyl glycerol is measured by the incorporation of radioactive serine and α-glycerol-P into the membrane phospholipids. Their incorporation requires CTP and Mn2+, and is stimulated by ATP and monovalent cations. The rate of incorporation of serine is not dependent on the transport of serine into the intravesicular space. When hydroxylamine is used to prevent decarboxylation of newly synthesized phosphatidyl serine, the serine moiety can exchange with exogenous serine.

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