GATA4 regulates estrogen receptor-α-mediated osteoblast transcription

Gustavo Miranda-Carboni, Miriam Guemes, Shannon Bailey, Edgar Anaya, Mirko Corselli, Bruno Peault, Susan Miranda

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Estrogens regulate osteoblast differentiation and mineralization. We identified GATA4 as a transcription factor expressed in osteoblasts and directly regulated by 17β-estradiol in this cell type but not in breast cancer cells, another estrogen-responsive tissue. Chromatin immunoprecipitation sequencing (chromatin immunoprecipitation sequencing) reveals that estrogen receptor α (ERα) binds to chromatin near GATA4 at five different enhancers. GATA4 and ERα are both recruited to ERα binding sites near genes that are specifically expressed in osteoblasts and control osteoblast differentiation. Maximal binding of GATA4 precedes ERα binding, and GATA4 is necessary for histone 3 lysine 4 dimethylation at ERα binding sites, suggesting that GATA4 is a pioneer factor for ERα. As such, knockdown of GATA4 reduced recruitment of ERα to DNA. Our study illustrates that GATA4 is a pioneer factor for ERα recruitment to osteoblastspecific enhancers.

Original languageEnglish (US)
Pages (from-to)1126-1136
Number of pages11
JournalMolecular Endocrinology
Volume25
Issue number7
DOIs
StatePublished - Jul 1 2011

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Osteoblasts
Estrogen Receptors
Chromatin Immunoprecipitation
Estrogens
Binding Sites
Histones
Lysine
Chromatin
Estradiol
Transcription Factors
Breast Neoplasms
DNA
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Endocrinology

Cite this

GATA4 regulates estrogen receptor-α-mediated osteoblast transcription. / Miranda-Carboni, Gustavo; Guemes, Miriam; Bailey, Shannon; Anaya, Edgar; Corselli, Mirko; Peault, Bruno; Miranda, Susan.

In: Molecular Endocrinology, Vol. 25, No. 7, 01.07.2011, p. 1126-1136.

Research output: Contribution to journalArticle

Miranda-Carboni, Gustavo ; Guemes, Miriam ; Bailey, Shannon ; Anaya, Edgar ; Corselli, Mirko ; Peault, Bruno ; Miranda, Susan. / GATA4 regulates estrogen receptor-α-mediated osteoblast transcription. In: Molecular Endocrinology. 2011 ; Vol. 25, No. 7. pp. 1126-1136.
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