Gene expression profiling and network analysis of peripheral blood monocytes in a chronic model of allergic asthma

Glenn P. Dorsam, Scott A. Hoselton, Ashley R. Sandy, Amali Samarasinghe, Emilie E. Vomhof-DeKrey, Sheri T. Dorsam, Jane M. Schuh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The Aspergillus fumigatus mouse model of asthma mimics the characteristics of human fungal asthma, including local and systemic inflammation. Monocyte/macrophage lineage cells direct innate immune responses and guide adaptive responses. To identify gene expression changes in peripheral blood mono-cytes in the context of fungal allergy, mice were exposed to systemic and intranasal inoculations of fungal antigen (sensitized), and naïve and sensitized animals were challenged intratracheally with live A. fumigatus conidia. Microarray analysis of blood monocytes from allergic versus non-allergic mice showed ≥ twofold modulation of 45 genes. Ingenuity pathway analysis revealed a network of these genes involved in antigen presentation, inflammation, and immune cell trafficking. These data show that allergen sensitization and challenge affects gene expression in peripheral monocytes.

Original languageEnglish (US)
Pages (from-to)558-563
Number of pages6
JournalMicrobiology and immunology
Volume54
Issue number9
DOIs
StatePublished - Jan 1 2010

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Gene Regulatory Networks
Gene Expression Profiling
Monocytes
Aspergillus fumigatus
Asthma
Fungal Antigens
Inflammation
Gene Expression
Fungal Spores
Antigen Presentation
Microarray Analysis
Innate Immunity
Allergens
Hypersensitivity
Macrophages
Genes

All Science Journal Classification (ASJC) codes

  • Immunology
  • Microbiology
  • Virology
  • Medicine(all)

Cite this

Gene expression profiling and network analysis of peripheral blood monocytes in a chronic model of allergic asthma. / Dorsam, Glenn P.; Hoselton, Scott A.; Sandy, Ashley R.; Samarasinghe, Amali; Vomhof-DeKrey, Emilie E.; Dorsam, Sheri T.; Schuh, Jane M.

In: Microbiology and immunology, Vol. 54, No. 9, 01.01.2010, p. 558-563.

Research output: Contribution to journalArticle

Dorsam, Glenn P. ; Hoselton, Scott A. ; Sandy, Ashley R. ; Samarasinghe, Amali ; Vomhof-DeKrey, Emilie E. ; Dorsam, Sheri T. ; Schuh, Jane M. / Gene expression profiling and network analysis of peripheral blood monocytes in a chronic model of allergic asthma. In: Microbiology and immunology. 2010 ; Vol. 54, No. 9. pp. 558-563.
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