Gene expression profiling of Mycobacterium tuberculosis Lipoarabinomannan-treated macrophages

A role of the Bcl-2 family member A1 in inhibition of apoptosis in mycobacteria-infected macrophages

Priyanka Halder, Ranjeet Kumar, Kuladip Jana, Sohini Chakraborty, Zhumur Ghosh, Manikuntala Kundu, Joyoti Basu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Macrophages play an important role in the establishment of infection by intracellular pathogens. Mycobacterium tuberculosis is known to inhibit apoptosis and to downregulate immune responses of host cells using various strategies, including activation of peroxisome proliferator-activated receptor (PPAR)γ. Mannose-capped lipoarabinomannan (ManLAM) is one of the known bacterial effectors that plays a role in subversion of host immunity and activation of PPARγ. Here, we have used an unbiased global gene expression profiling approach to understand (a) how ManLAM regulates host cell immune responses and (b) the role of PPARγ in modulating ManLAM-induced host cell signaling. We have demonstrated that ManLAM-dependent inhibition of macrophage apoptosis is mediated by the upregulation of the antiapoptotic B-cell CLL/lymphoma 2 (Bcl2) family member A1. Our in silico analyses suggested that ManLAM-mediated PPARγ signaling is linked to important functions such as phagocytosis, cytoskeleton remodeling, cell survival, and autophagy. We have validated that ManLAM upregulates signal transducer and activator of transcription (STAT5)α, an important transcriptional regulator of cell survival in a PPARγ-dependent manner.

Original languageEnglish (US)
Pages (from-to)726-736
Number of pages11
JournalIUBMB Life
Volume67
Issue number9
DOIs
StatePublished - Sep 1 2015

Fingerprint

Macrophages
Gene Expression Profiling
Mycobacterium
Mannose
Peroxisome Proliferator-Activated Receptors
Mycobacterium tuberculosis
Gene expression
Apoptosis
Cells
Cell Survival
Up-Regulation
Chemical activation
Cell signaling
Autophagy
B-Cell Lymphoma
Pathogens
Transcription
Cytoskeleton
Transducers
Phagocytosis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

Cite this

Gene expression profiling of Mycobacterium tuberculosis Lipoarabinomannan-treated macrophages : A role of the Bcl-2 family member A1 in inhibition of apoptosis in mycobacteria-infected macrophages. / Halder, Priyanka; Kumar, Ranjeet; Jana, Kuladip; Chakraborty, Sohini; Ghosh, Zhumur; Kundu, Manikuntala; Basu, Joyoti.

In: IUBMB Life, Vol. 67, No. 9, 01.09.2015, p. 726-736.

Research output: Contribution to journalArticle

Halder, Priyanka ; Kumar, Ranjeet ; Jana, Kuladip ; Chakraborty, Sohini ; Ghosh, Zhumur ; Kundu, Manikuntala ; Basu, Joyoti. / Gene expression profiling of Mycobacterium tuberculosis Lipoarabinomannan-treated macrophages : A role of the Bcl-2 family member A1 in inhibition of apoptosis in mycobacteria-infected macrophages. In: IUBMB Life. 2015 ; Vol. 67, No. 9. pp. 726-736.
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