Gene expression profiling of the response of Streptococcus pneumoniae to penicillin

Phillip Rogers, Teresa T. Liu, Katherine S. Barker, George M. Hilliard, B. Keith English, Justin Thornton, Edwin Swiatlo, Larry S. McDaniel

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Objectives: The aim of this study was to identify changes in the gene expression profile of Streptococcus pneumoniae in response to a subinhibitory concentration of penicillin in an effort to better understand mechanisms by which this organism copes with this stress. Methods: S. pneumoniae serotype 2 strain D39 was grown for 1 h in the presence or absence of penicillin at a concentration equivalent to half the MIC (0.03 mg/L). RNA was isolated and gene expression profiles were compared using DNA microarrays. Differential expression of select genes was confirmed by real-time RT-PCR. Results: A total of 386 genes were found to be responsive to penicillin. Up-regulated genes included those of the ciaR - ciaH operon, luxS, genes encoding cell envelope proteins and genes of the pst locus. Down-regulated genes included genes involved in competence, genes encoding capsular polysaccharide biosynthesis proteins, genes involved in fatty acid chain elongation and genes of the polyamine transporter operon. Conclusions: Altered expression of these genes reflects a protective response to perturbation of the bacterial cell wall by penicillin. Such genes may represent potential therapeutic targets for enhancing the activity of penicillin against this organism and provide insight into novel mechanisms of penicillin resistance.

Original languageEnglish (US)
Pages (from-to)616-626
Number of pages11
JournalJournal of Antimicrobial Chemotherapy
Volume59
Issue number4
DOIs
StatePublished - Apr 1 2007

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Gene Expression Profiling
Streptococcus pneumoniae
Penicillins
Genes
Operon
Transcriptome
Penicillin Resistance
Gene Expression
Polyamines
Protein Biosynthesis
Oligonucleotide Array Sequence Analysis
Mental Competency
Cell Wall
Polysaccharides
Real-Time Polymerase Chain Reaction
Proteins
Fatty Acids
RNA

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Rogers, P., Liu, T. T., Barker, K. S., Hilliard, G. M., English, B. K., Thornton, J., ... McDaniel, L. S. (2007). Gene expression profiling of the response of Streptococcus pneumoniae to penicillin. Journal of Antimicrobial Chemotherapy, 59(4), 616-626. https://doi.org/10.1093/jac/dkl560

Gene expression profiling of the response of Streptococcus pneumoniae to penicillin. / Rogers, Phillip; Liu, Teresa T.; Barker, Katherine S.; Hilliard, George M.; English, B. Keith; Thornton, Justin; Swiatlo, Edwin; McDaniel, Larry S.

In: Journal of Antimicrobial Chemotherapy, Vol. 59, No. 4, 01.04.2007, p. 616-626.

Research output: Contribution to journalArticle

Rogers, P, Liu, TT, Barker, KS, Hilliard, GM, English, BK, Thornton, J, Swiatlo, E & McDaniel, LS 2007, 'Gene expression profiling of the response of Streptococcus pneumoniae to penicillin', Journal of Antimicrobial Chemotherapy, vol. 59, no. 4, pp. 616-626. https://doi.org/10.1093/jac/dkl560
Rogers, Phillip ; Liu, Teresa T. ; Barker, Katherine S. ; Hilliard, George M. ; English, B. Keith ; Thornton, Justin ; Swiatlo, Edwin ; McDaniel, Larry S. / Gene expression profiling of the response of Streptococcus pneumoniae to penicillin. In: Journal of Antimicrobial Chemotherapy. 2007 ; Vol. 59, No. 4. pp. 616-626.
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