Gene profiling the effects of calcium deficiency versus 1,25-dihydroxyvitamin D induced hypercalcemia in rat kidney cortex

Amandeep Bajwa, R. L. Horst, M. J. Beckman

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Determinants involved in the activation and repression of 1,25-dihydroxyvitamin D (1,25(OH)2D3) synthesis in renal cortex by changes in extracellular Ca were studied. Cortical kidney RNA isolated from hypocalcemic (LC) rats generated by a low Ca diet, and hypercalcemic (HC) rats generated by a normal Ca diet and two sequential 1 μg doses of 1,25(OH)2D3. Among the genes up-regulated were 1α-OHase (4.6-fold) in the LC group and high differential gene expression of VDR (4.0-fold) and 24-OHase (10.4-fold) in the HC group. Moreover, the exposure of renal cortex to LC versus HC conditions revealed a high differential expression of a PKA-dominated pathway involving CBP interacting protein, GATA-1 and CREB transcription factors in the LC model. In the HC model, elevated renal cortex gene expression of several growth factors, peptide receptors, and intracellular signaling molecules depicts a role for CaSR activation and receptor tyrosine kinase signaling in 1,25(OH)2D3-mediated gene activation and repression of 1α-OHase.

Original languageEnglish (US)
Pages (from-to)182-194
Number of pages13
JournalArchives of Biochemistry and Biophysics
Volume438
Issue number2
DOIs
StatePublished - Jun 15 2005

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Kidney Cortex
Hypercalcemia
Rats
Genes
Chemical activation
Nutrition
Calcium
Kidney
Gene expression
Peptide Receptors
Receptor Protein-Tyrosine Kinases
Diet
Gene Expression
Growth Factor Receptors
Intercellular Signaling Peptides and Proteins
Transcription Factors
RNA
Transcriptional Activation
Molecules
1,25-dihydroxyvitamin D

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Gene profiling the effects of calcium deficiency versus 1,25-dihydroxyvitamin D induced hypercalcemia in rat kidney cortex. / Bajwa, Amandeep; Horst, R. L.; Beckman, M. J.

In: Archives of Biochemistry and Biophysics, Vol. 438, No. 2, 15.06.2005, p. 182-194.

Research output: Contribution to journalArticle

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