Gene transfer and expression of human erythropoietin in mice in vivo

Junming Yue, Bao Quan Jiao, Bu Nian Jia, Rong Liang Hu, Ning Yi Jin, Zhen Yin, Jiang Shi, Hai Yang Nie, Yun Long Hu, Gang Wu, Zong An Zhou

Research output: Contribution to journalArticle

Abstract

The expression vector was successfully constructed with the cloning of ery-thropoietin genomic DNA into the downstream of CMV promoter, and expressed in transfected transiently BHK21 cells. Then it was introduced into the skeletal muscle of mice by the mediation of lipofectin. As a result, the EPO expressed in serum of mice reached highly to 1 340 ng/L in one month.

Original languageEnglish (US)
Pages (from-to)70-71
Number of pages2
JournalProgress in Biochemistry and Biophysics
Volume25
Issue number1
StatePublished - Dec 1 1998
Externally publishedYes

Fingerprint

Gene transfer
Cloning
Erythropoietin
Gene expression
Muscle
Gene Expression
DNA
Organism Cloning
Skeletal Muscle
Serum
1,2-dielaidoylphosphatidylethanolamine

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry

Cite this

Yue, J., Jiao, B. Q., Jia, B. N., Hu, R. L., Jin, N. Y., Yin, Z., ... Zhou, Z. A. (1998). Gene transfer and expression of human erythropoietin in mice in vivo. Progress in Biochemistry and Biophysics, 25(1), 70-71.

Gene transfer and expression of human erythropoietin in mice in vivo. / Yue, Junming; Jiao, Bao Quan; Jia, Bu Nian; Hu, Rong Liang; Jin, Ning Yi; Yin, Zhen; Shi, Jiang; Nie, Hai Yang; Hu, Yun Long; Wu, Gang; Zhou, Zong An.

In: Progress in Biochemistry and Biophysics, Vol. 25, No. 1, 01.12.1998, p. 70-71.

Research output: Contribution to journalArticle

Yue, J, Jiao, BQ, Jia, BN, Hu, RL, Jin, NY, Yin, Z, Shi, J, Nie, HY, Hu, YL, Wu, G & Zhou, ZA 1998, 'Gene transfer and expression of human erythropoietin in mice in vivo', Progress in Biochemistry and Biophysics, vol. 25, no. 1, pp. 70-71.
Yue, Junming ; Jiao, Bao Quan ; Jia, Bu Nian ; Hu, Rong Liang ; Jin, Ning Yi ; Yin, Zhen ; Shi, Jiang ; Nie, Hai Yang ; Hu, Yun Long ; Wu, Gang ; Zhou, Zong An. / Gene transfer and expression of human erythropoietin in mice in vivo. In: Progress in Biochemistry and Biophysics. 1998 ; Vol. 25, No. 1. pp. 70-71.
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