Generation and characterization of a recombinant vesicular stomatitis virus expressing the glycoprotein of Borna disease virus

Mar Perez, Roberto Clemente, Clinton S. Robison, E. Jeetendra, Himangi R. Jayakar, Michael Whitt, Juan C. De La Torre

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Borna disease virus (BDV) is an enveloped virus with a nonsegmented negative-strand RNA genome whose organization is characteristic of mononegavirales. However, based on its unique genetics and biological features, BDV is considered to be the prototypic member of a new virus family, Bornaviridae, within the order Mononegavirales. BDV cell entry occurs via receptor-mediated endocytosis, a process initiated by the recognition of an as yet unidentified receptor at the cell surface by the BDV surface glycoprotein (G). The paucity of cell-free virus associated with BDV infection has hindered studies aimed at the elucidation of cellular receptors and detailed mechanisms involved in BDV cell entry. To overcome this problem, we generated and characterized a replication-competent recombinant vesicular stomatitis virus expressing BDV G (rVSVAG*/BDVG). Cells infected with rVSVAG*/BDVG produced high titers (107 PFU/ml) of cell-free virus progeny, but this virus exhibited a highly attenuated phenotype both in cell culture and in vivo. Attenuation of rVSVAG*/BDVG was associated with a delayed kinetics of viral RNA replication and altered genome/N mRNA ratios compared to results for rVSVAG*/VSVG. Likewise, incorporation of BDV G into virions appeared to be restricted despite its high levels of expression and efficient processing in rVSVAG*/BDVG-infected cells. Notably, rVSVAG*/BDVG recreated the cell tropism and entry pathway of bona fide BDV. Our results indicate that rVSVAG*/BDVG represents a unique tool for the investigation of BDV G-mediated cell entry, as well as the roles of BDV G in host immune responses and pathogenesis associated with BDV infection.

Original languageEnglish (US)
Pages (from-to)5527-5536
Number of pages10
JournalJournal of Virology
Volume81
Issue number11
DOIs
StatePublished - Jun 1 2007

Fingerprint

Borna disease virus
Vesiculovirus
Vesicular Stomatitis
glycoproteins
Glycoproteins
Viruses
Mononegavirales
cells
viruses
Virus Internalization
Virus Diseases
receptors
Bornaviridae
Genome
Gastrin-Secreting Cells
tropisms
Tropism
genome
Membrane Glycoproteins
Viral RNA

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Generation and characterization of a recombinant vesicular stomatitis virus expressing the glycoprotein of Borna disease virus. / Perez, Mar; Clemente, Roberto; Robison, Clinton S.; Jeetendra, E.; Jayakar, Himangi R.; Whitt, Michael; De La Torre, Juan C.

In: Journal of Virology, Vol. 81, No. 11, 01.06.2007, p. 5527-5536.

Research output: Contribution to journalArticle

Perez, Mar ; Clemente, Roberto ; Robison, Clinton S. ; Jeetendra, E. ; Jayakar, Himangi R. ; Whitt, Michael ; De La Torre, Juan C. / Generation and characterization of a recombinant vesicular stomatitis virus expressing the glycoprotein of Borna disease virus. In: Journal of Virology. 2007 ; Vol. 81, No. 11. pp. 5527-5536.
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