Genes coding evolutionary novel anti-carbohydrate antibodies

Studies on anti-Gal production in α1,3galactosyltransferase knock out mice

Zhao Chun Chen, Marko Radic, Uri Galili

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

This study analyzes the gene repertoire coding for antibodies to an evolutionary novel immunogenic carbohydrate antigen in mice. The α-gal epitope (Galα1-3Galβ1-4GlcNAc-R) is an autoantigen, abundantly expressed in wild type mice, but absent in α1,3galactosyltransferase knock-out (KO) mice, where it can induce the production of the anti-Gal antibody. Hybridoma clones secreting anti-Gal were isolated from different mice and their immunoglobulin genes were analyzed. All anti-Gal clones were found to be encoded by the heavy chain gene VH22.1 and light chain gene VK5.1. Moreover, one 'forbidden' anti-Gal clone, produced in a wild type mouse, was also encoded by VH 22.1 and VK 5.1. The genes coding for the different anti-Gal clones were found to contain somatic mutations and different CDR3 domains. These data imply that a highly restricted gene usage combined with junctional diversity and somatic mutations can generate new antibodies that have not been produced in the course of the evolution of a species. (C) 2000 Elsevier Science Ltd.

Original languageEnglish (US)
Pages (from-to)455-466
Number of pages12
JournalMolecular Immunology
Volume37
Issue number8
DOIs
StatePublished - Dec 7 2000

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Knockout Mice
Anti-Idiotypic Antibodies
Carbohydrates
Clone Cells
Genes
Immunoglobulin Genes
Mutation
Antibodies
Autoantigens
Hybridomas
Epitopes
Light
Antigens

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

Cite this

Genes coding evolutionary novel anti-carbohydrate antibodies : Studies on anti-Gal production in α1,3galactosyltransferase knock out mice. / Chen, Zhao Chun; Radic, Marko; Galili, Uri.

In: Molecular Immunology, Vol. 37, No. 8, 07.12.2000, p. 455-466.

Research output: Contribution to journalArticle

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