Genetic analysis of iron-deficiency effects on the mouse spleen

Jennifer N. Gibson, Leslie C. Jellen, Erica L. Unger, Grant Morahan, Munish Mehta, Christopher J. Earley, Richard P. Allen, Lu Lu, Byron Jones

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Iron homeostasis is crucial to many biological functions in nearly all organisms, with roles ranging from oxygen transport to immune function. Disruption of iron homeostasis may result in iron overload or iron deficiency. Iron deficiency may have severe consequences, including anemia or changes in immune or neurotransmitter systems. Here we report on the variability of phenotypic iron tissue loss and splenomegaly and the associated quantitative trait loci (QTLs), polymorphic areas in the mouse genome that may contain one or more genes that play a role in spleen iron concentration or spleen weight under each dietary treatment. Mice from 26 BXD/Ty recombinant inbred strains, including the parent C57BL/6 and DBA/2 strains, were randomly assigned to adequate iron or iron-deficient diets at weaning. After 120 days, splenomegaly was measured by spleen weight, and spleen iron was assessed using a modified spectrophotometry technique. QTL analyses and gene expression comparisons were then conducted using the WebQTL GeneNetwork. We observed wide, genetic-based variability in splenomegaly and spleen iron loss in BXD/Ty recombinant inbred strains fed an iron-deficient diet. Moreover, we identified several suggestive QTLs. Matching our QTLs with gene expression data from the spleen revealed candidate genes. Our work shows that individual differences in splenomegaly response to iron deficiency are influenced at least partly by genetic constitution. We propose mechanistic hypotheses by which splenomegaly may result from iron deficiency.

Original languageEnglish (US)
Pages (from-to)556-562
Number of pages7
JournalMammalian Genome
Volume22
Issue number9-10
DOIs
StatePublished - Oct 1 2011

Fingerprint

Spleen
Iron
Splenomegaly
Quantitative Trait Loci
Homeostasis
Diet
Gene Expression
Weights and Measures
Iron Overload
Spectrophotometry
Constitution and Bylaws
Weaning
Individuality
Genes
Neurotransmitter Agents
Anemia
Genome
Oxygen

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Gibson, J. N., Jellen, L. C., Unger, E. L., Morahan, G., Mehta, M., Earley, C. J., ... Jones, B. (2011). Genetic analysis of iron-deficiency effects on the mouse spleen. Mammalian Genome, 22(9-10), 556-562. https://doi.org/10.1007/s00335-011-9344-4

Genetic analysis of iron-deficiency effects on the mouse spleen. / Gibson, Jennifer N.; Jellen, Leslie C.; Unger, Erica L.; Morahan, Grant; Mehta, Munish; Earley, Christopher J.; Allen, Richard P.; Lu, Lu; Jones, Byron.

In: Mammalian Genome, Vol. 22, No. 9-10, 01.10.2011, p. 556-562.

Research output: Contribution to journalArticle

Gibson, JN, Jellen, LC, Unger, EL, Morahan, G, Mehta, M, Earley, CJ, Allen, RP, Lu, L & Jones, B 2011, 'Genetic analysis of iron-deficiency effects on the mouse spleen', Mammalian Genome, vol. 22, no. 9-10, pp. 556-562. https://doi.org/10.1007/s00335-011-9344-4
Gibson JN, Jellen LC, Unger EL, Morahan G, Mehta M, Earley CJ et al. Genetic analysis of iron-deficiency effects on the mouse spleen. Mammalian Genome. 2011 Oct 1;22(9-10):556-562. https://doi.org/10.1007/s00335-011-9344-4
Gibson, Jennifer N. ; Jellen, Leslie C. ; Unger, Erica L. ; Morahan, Grant ; Mehta, Munish ; Earley, Christopher J. ; Allen, Richard P. ; Lu, Lu ; Jones, Byron. / Genetic analysis of iron-deficiency effects on the mouse spleen. In: Mammalian Genome. 2011 ; Vol. 22, No. 9-10. pp. 556-562.
@article{2c18e42168f84626ae2d26127acdef1e,
title = "Genetic analysis of iron-deficiency effects on the mouse spleen",
abstract = "Iron homeostasis is crucial to many biological functions in nearly all organisms, with roles ranging from oxygen transport to immune function. Disruption of iron homeostasis may result in iron overload or iron deficiency. Iron deficiency may have severe consequences, including anemia or changes in immune or neurotransmitter systems. Here we report on the variability of phenotypic iron tissue loss and splenomegaly and the associated quantitative trait loci (QTLs), polymorphic areas in the mouse genome that may contain one or more genes that play a role in spleen iron concentration or spleen weight under each dietary treatment. Mice from 26 BXD/Ty recombinant inbred strains, including the parent C57BL/6 and DBA/2 strains, were randomly assigned to adequate iron or iron-deficient diets at weaning. After 120 days, splenomegaly was measured by spleen weight, and spleen iron was assessed using a modified spectrophotometry technique. QTL analyses and gene expression comparisons were then conducted using the WebQTL GeneNetwork. We observed wide, genetic-based variability in splenomegaly and spleen iron loss in BXD/Ty recombinant inbred strains fed an iron-deficient diet. Moreover, we identified several suggestive QTLs. Matching our QTLs with gene expression data from the spleen revealed candidate genes. Our work shows that individual differences in splenomegaly response to iron deficiency are influenced at least partly by genetic constitution. We propose mechanistic hypotheses by which splenomegaly may result from iron deficiency.",
author = "Gibson, {Jennifer N.} and Jellen, {Leslie C.} and Unger, {Erica L.} and Grant Morahan and Munish Mehta and Earley, {Christopher J.} and Allen, {Richard P.} and Lu Lu and Byron Jones",
year = "2011",
month = "10",
day = "1",
doi = "10.1007/s00335-011-9344-4",
language = "English (US)",
volume = "22",
pages = "556--562",
journal = "Mammalian Genome",
issn = "0938-8990",
publisher = "Springer New York",
number = "9-10",

}

TY - JOUR

T1 - Genetic analysis of iron-deficiency effects on the mouse spleen

AU - Gibson, Jennifer N.

AU - Jellen, Leslie C.

AU - Unger, Erica L.

AU - Morahan, Grant

AU - Mehta, Munish

AU - Earley, Christopher J.

AU - Allen, Richard P.

AU - Lu, Lu

AU - Jones, Byron

PY - 2011/10/1

Y1 - 2011/10/1

N2 - Iron homeostasis is crucial to many biological functions in nearly all organisms, with roles ranging from oxygen transport to immune function. Disruption of iron homeostasis may result in iron overload or iron deficiency. Iron deficiency may have severe consequences, including anemia or changes in immune or neurotransmitter systems. Here we report on the variability of phenotypic iron tissue loss and splenomegaly and the associated quantitative trait loci (QTLs), polymorphic areas in the mouse genome that may contain one or more genes that play a role in spleen iron concentration or spleen weight under each dietary treatment. Mice from 26 BXD/Ty recombinant inbred strains, including the parent C57BL/6 and DBA/2 strains, were randomly assigned to adequate iron or iron-deficient diets at weaning. After 120 days, splenomegaly was measured by spleen weight, and spleen iron was assessed using a modified spectrophotometry technique. QTL analyses and gene expression comparisons were then conducted using the WebQTL GeneNetwork. We observed wide, genetic-based variability in splenomegaly and spleen iron loss in BXD/Ty recombinant inbred strains fed an iron-deficient diet. Moreover, we identified several suggestive QTLs. Matching our QTLs with gene expression data from the spleen revealed candidate genes. Our work shows that individual differences in splenomegaly response to iron deficiency are influenced at least partly by genetic constitution. We propose mechanistic hypotheses by which splenomegaly may result from iron deficiency.

AB - Iron homeostasis is crucial to many biological functions in nearly all organisms, with roles ranging from oxygen transport to immune function. Disruption of iron homeostasis may result in iron overload or iron deficiency. Iron deficiency may have severe consequences, including anemia or changes in immune or neurotransmitter systems. Here we report on the variability of phenotypic iron tissue loss and splenomegaly and the associated quantitative trait loci (QTLs), polymorphic areas in the mouse genome that may contain one or more genes that play a role in spleen iron concentration or spleen weight under each dietary treatment. Mice from 26 BXD/Ty recombinant inbred strains, including the parent C57BL/6 and DBA/2 strains, were randomly assigned to adequate iron or iron-deficient diets at weaning. After 120 days, splenomegaly was measured by spleen weight, and spleen iron was assessed using a modified spectrophotometry technique. QTL analyses and gene expression comparisons were then conducted using the WebQTL GeneNetwork. We observed wide, genetic-based variability in splenomegaly and spleen iron loss in BXD/Ty recombinant inbred strains fed an iron-deficient diet. Moreover, we identified several suggestive QTLs. Matching our QTLs with gene expression data from the spleen revealed candidate genes. Our work shows that individual differences in splenomegaly response to iron deficiency are influenced at least partly by genetic constitution. We propose mechanistic hypotheses by which splenomegaly may result from iron deficiency.

UR - http://www.scopus.com/inward/record.url?scp=80054767995&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054767995&partnerID=8YFLogxK

U2 - 10.1007/s00335-011-9344-4

DO - 10.1007/s00335-011-9344-4

M3 - Article

C2 - 21732193

AN - SCOPUS:80054767995

VL - 22

SP - 556

EP - 562

JO - Mammalian Genome

JF - Mammalian Genome

SN - 0938-8990

IS - 9-10

ER -