Genetic analysis of tongue size and taste papillae number and size in recombinant inbred strains of mice

David J. Reiner, Taha A. Jan, John Boughter, Cheng Xiang Li, Lu Lu, Robert Williams, Robert Waters

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Quantitative trait loci (QTLs) analysis has been used to examine natural variation of phenotypes in the mouse somatosensory cortex, hippocampus, cerebellum, and amygdala. QTL analysis has also been utilized to map and identify genes underlying anatomical features such as muscle, organ, and body weights. However, this methodology has not been previously applied to identification of anatomical structures related to gustatory phenotypes. In this study, we used QTL analysis to map and characterize genes underlying tongue size, papillae number, and papillae area. In a set of 43 BXD recombinant inbred (RI) mice (n = 111) and 2 parental strains (C57BL/6J and DBA/2J; n = 7), we measured tongue length, width, and weight. In a subset of 23 BXD RI mice and the parental mice, we measured filiform and fungiform papillae number and fungiform papillae area. Using QTL linkage analysis (through WebQTL), we detected 2 significant and noninteracting QTLs influencing tongue length on chromosomes 5 and 7. We also found a significant QTL on chromosome 19 underlying fungiform papillae area and a suggestive QTL on chromosome 2 linked to fungiform papillae number. From these QTLs, we identified a number of candidate genes within the QTL intervals that include SRY-box containing gene, nebulin-related anchoring protein, and actin-binding LIM protein 1. This study is an important first step in identifying genetic factors underlying tongue size, papillae size, and papillae number using QTL analysis.

Original languageEnglish (US)
Pages (from-to)693-707
Number of pages15
JournalChemical senses
Volume33
Issue number8
DOIs
StatePublished - Oct 1 2008

Fingerprint

Inbred Strains Mice
Quantitative Trait Loci
Tongue
Genes
Phenotype
Chromosomes, Human, Pair 19
Chromosomes, Human, Pair 5
Chromosomes, Human, Pair 7
Somatosensory Cortex
Chromosomes, Human, Pair 2
Organ Size
Amygdala
Cerebellum
Actins
Hippocampus
Carrier Proteins
Body Weight
Weights and Measures
Muscles

All Science Journal Classification (ASJC) codes

  • Physiology
  • Sensory Systems
  • Physiology (medical)
  • Behavioral Neuroscience

Cite this

Genetic analysis of tongue size and taste papillae number and size in recombinant inbred strains of mice. / Reiner, David J.; Jan, Taha A.; Boughter, John; Li, Cheng Xiang; Lu, Lu; Williams, Robert; Waters, Robert.

In: Chemical senses, Vol. 33, No. 8, 01.10.2008, p. 693-707.

Research output: Contribution to journalArticle

@article{b57b4e94f7f2449885f355199d1c79e6,
title = "Genetic analysis of tongue size and taste papillae number and size in recombinant inbred strains of mice",
abstract = "Quantitative trait loci (QTLs) analysis has been used to examine natural variation of phenotypes in the mouse somatosensory cortex, hippocampus, cerebellum, and amygdala. QTL analysis has also been utilized to map and identify genes underlying anatomical features such as muscle, organ, and body weights. However, this methodology has not been previously applied to identification of anatomical structures related to gustatory phenotypes. In this study, we used QTL analysis to map and characterize genes underlying tongue size, papillae number, and papillae area. In a set of 43 BXD recombinant inbred (RI) mice (n = 111) and 2 parental strains (C57BL/6J and DBA/2J; n = 7), we measured tongue length, width, and weight. In a subset of 23 BXD RI mice and the parental mice, we measured filiform and fungiform papillae number and fungiform papillae area. Using QTL linkage analysis (through WebQTL), we detected 2 significant and noninteracting QTLs influencing tongue length on chromosomes 5 and 7. We also found a significant QTL on chromosome 19 underlying fungiform papillae area and a suggestive QTL on chromosome 2 linked to fungiform papillae number. From these QTLs, we identified a number of candidate genes within the QTL intervals that include SRY-box containing gene, nebulin-related anchoring protein, and actin-binding LIM protein 1. This study is an important first step in identifying genetic factors underlying tongue size, papillae size, and papillae number using QTL analysis.",
author = "Reiner, {David J.} and Jan, {Taha A.} and John Boughter and Li, {Cheng Xiang} and Lu Lu and Robert Williams and Robert Waters",
year = "2008",
month = "10",
day = "1",
doi = "10.1093/chemse/bjn025",
language = "English (US)",
volume = "33",
pages = "693--707",
journal = "Chemical Senses",
issn = "0379-864X",
publisher = "Oxford University Press",
number = "8",

}

TY - JOUR

T1 - Genetic analysis of tongue size and taste papillae number and size in recombinant inbred strains of mice

AU - Reiner, David J.

AU - Jan, Taha A.

AU - Boughter, John

AU - Li, Cheng Xiang

AU - Lu, Lu

AU - Williams, Robert

AU - Waters, Robert

PY - 2008/10/1

Y1 - 2008/10/1

N2 - Quantitative trait loci (QTLs) analysis has been used to examine natural variation of phenotypes in the mouse somatosensory cortex, hippocampus, cerebellum, and amygdala. QTL analysis has also been utilized to map and identify genes underlying anatomical features such as muscle, organ, and body weights. However, this methodology has not been previously applied to identification of anatomical structures related to gustatory phenotypes. In this study, we used QTL analysis to map and characterize genes underlying tongue size, papillae number, and papillae area. In a set of 43 BXD recombinant inbred (RI) mice (n = 111) and 2 parental strains (C57BL/6J and DBA/2J; n = 7), we measured tongue length, width, and weight. In a subset of 23 BXD RI mice and the parental mice, we measured filiform and fungiform papillae number and fungiform papillae area. Using QTL linkage analysis (through WebQTL), we detected 2 significant and noninteracting QTLs influencing tongue length on chromosomes 5 and 7. We also found a significant QTL on chromosome 19 underlying fungiform papillae area and a suggestive QTL on chromosome 2 linked to fungiform papillae number. From these QTLs, we identified a number of candidate genes within the QTL intervals that include SRY-box containing gene, nebulin-related anchoring protein, and actin-binding LIM protein 1. This study is an important first step in identifying genetic factors underlying tongue size, papillae size, and papillae number using QTL analysis.

AB - Quantitative trait loci (QTLs) analysis has been used to examine natural variation of phenotypes in the mouse somatosensory cortex, hippocampus, cerebellum, and amygdala. QTL analysis has also been utilized to map and identify genes underlying anatomical features such as muscle, organ, and body weights. However, this methodology has not been previously applied to identification of anatomical structures related to gustatory phenotypes. In this study, we used QTL analysis to map and characterize genes underlying tongue size, papillae number, and papillae area. In a set of 43 BXD recombinant inbred (RI) mice (n = 111) and 2 parental strains (C57BL/6J and DBA/2J; n = 7), we measured tongue length, width, and weight. In a subset of 23 BXD RI mice and the parental mice, we measured filiform and fungiform papillae number and fungiform papillae area. Using QTL linkage analysis (through WebQTL), we detected 2 significant and noninteracting QTLs influencing tongue length on chromosomes 5 and 7. We also found a significant QTL on chromosome 19 underlying fungiform papillae area and a suggestive QTL on chromosome 2 linked to fungiform papillae number. From these QTLs, we identified a number of candidate genes within the QTL intervals that include SRY-box containing gene, nebulin-related anchoring protein, and actin-binding LIM protein 1. This study is an important first step in identifying genetic factors underlying tongue size, papillae size, and papillae number using QTL analysis.

UR - http://www.scopus.com/inward/record.url?scp=54949091894&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=54949091894&partnerID=8YFLogxK

U2 - 10.1093/chemse/bjn025

DO - 10.1093/chemse/bjn025

M3 - Article

C2 - 18653645

AN - SCOPUS:54949091894

VL - 33

SP - 693

EP - 707

JO - Chemical Senses

JF - Chemical Senses

SN - 0379-864X

IS - 8

ER -