Genetic and structural evidence for antigen selection of anti-DNA antibodies

Marko Radic, M. Weigert

Research output: Contribution to journalReview article

404 Citations (Scopus)

Abstract

The primary structure of anti-DNA antibodies is highly diverse, a result of different germline variable (V) gene use, different combinations of immunoglobulin gene segments, peculiar heavy chain complementarity determining region 3 (H-CDR3) segments, and somatic mutations. Nevertheless, tertiary structure predictions reveal common features that yield information about likely contact sites in the anti-DNA combining site. That these contacts are involved with DNA binding is supported by recurrent features of a newly compiled set of homology groups of 13 variable regions of heavy chains (V(H)) and 11 variable regions of light chains (VL), characteristic pattern of somatic mutations, and the results of site-directed mutagenesis. The role of antigen in the etiology of the autoimmune response is viewed in light of recent data on overlaps between anti-DNA and anti-nucleic acid binding protein specificities.

Original languageEnglish (US)
Pages (from-to)487-520
Number of pages34
JournalAnnual Review of Immunology
Volume12
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Fingerprint

Antinuclear Antibodies
Antigens
DNA
Complementarity Determining Regions
Immunoglobulin Genes
Mutation
Site-Directed Mutagenesis
Autoimmunity
Nucleic Acids
Carrier Proteins
Binding Sites
Light
Genes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Genetic and structural evidence for antigen selection of anti-DNA antibodies. / Radic, Marko; Weigert, M.

In: Annual Review of Immunology, Vol. 12, 01.01.1994, p. 487-520.

Research output: Contribution to journalReview article

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