Genetic control of the mouse cerebellum: Identification of quantitative trait loci modulating size and architecture

D. C. Airey, Lu Lu, Robert Williams

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

To discover genes influencing cerebellum development, we conducted a complex trait analysis of variation in the size of the adult mouse cerebellum. We analyzed two sets of recombinant inbred BXD strains and an F2 intercross of the common inbred strains, C57BL/6J and DBA/2J. We measured cerebellar size as the weight or volume of fixed or histologically processed tissue. Among BXD recombinant inbred strains, the cerebellum averages 52 mg (12.4% of the brain) and ranges 18 mg in size. In F2 mice, the cerebellum averages 62 mg (12.9% of the brain) and ranges ∼20 mg in size. Five quantitative trait loci (QTLs) that significantly control variation in cerebellar size were mapped to chromosomes 1 (Cbs1a), 8 (Cbs8a), 14 (Cbs14a), and 19 (Cbs19a, Cbs19b). In combination, these QTLs can shift cerebellar size an appreciable 35% of the observed range. To assess regional genetic control of the cerebellum, we also measured the volume of the cell-rich, internal granule layer (IGL) in a set of BXD strains. The IGL ranges from 34 to 43% of total cerebellar volume. The QTL Cbs8a is significantly linked to variation in IGL volume and is suggestively linked to variation in the number of cerebellar folia. The QTLs we have discovered are among the first loci shown to modulate the size and architecture of the adult mouse cerebellum.

Original languageEnglish (US)
Pages (from-to)5099-5109
Number of pages11
JournalJournal of Neuroscience
Volume21
Issue number14
StatePublished - Jul 15 2001

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Quantitative Trait Loci
Cerebellum
Internal-External Control
Chromosomes, Human, Pair 1
Brain
Cell Size
Weights and Measures
Genes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Genetic control of the mouse cerebellum : Identification of quantitative trait loci modulating size and architecture. / Airey, D. C.; Lu, Lu; Williams, Robert.

In: Journal of Neuroscience, Vol. 21, No. 14, 15.07.2001, p. 5099-5109.

Research output: Contribution to journalArticle

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