Genetic pathways regulating glutamate levels in retinal müller cells

Monica Jablonski, Natalie E. Freeman, William E. Orr, Justin P. Templeton, Lu Lu, Robert Williams, Eldon E. Geisert

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Müller cells serve many functions including the regulation of extracellular glutamate levels. The product of two genes, Slc1a3 [aka solute carrier family 1 (glial high affinity glutamate transporter), member 3] and Glul (aka glutamine synthetase) are the primary role players that transport glutamate into the Müller cell and convert it into glutamine. In this study, we sought to identify the genetic regulation of both genes. Given their tightly coupled biological functions, we predicted that they would be similarly regulated. Using an array of 75 recombinant inbred strains of mice, we determined that Slc1a3 and Glul are differentially regulated by distinct chromosomal regions. Interestingly, despite their independent regulation, gene ontology analysis of tightly correlated genes reveals that the enriched and statistically significant molecular function categories of both directed acyclic graphs have substantial overlap, indicating that the shared functions of correlates of Slc1a3 and Glul include production and usage of ATP.

Original languageEnglish (US)
Pages (from-to)594-603
Number of pages10
JournalNeurochemical Research
Volume36
Issue number4
DOIs
StatePublished - Apr 1 2011

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Glutamic Acid
Genes
Amino Acid Transport System X-AG
Glutamate-Ammonia Ligase
Gene Ontology
Inbred Strains Mice
Glutamine
Neuroglia
Adenosine Triphosphate
Gene expression
Ontology

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Genetic pathways regulating glutamate levels in retinal müller cells. / Jablonski, Monica; Freeman, Natalie E.; Orr, William E.; Templeton, Justin P.; Lu, Lu; Williams, Robert; Geisert, Eldon E.

In: Neurochemical Research, Vol. 36, No. 4, 01.04.2011, p. 594-603.

Research output: Contribution to journalArticle

Jablonski, Monica ; Freeman, Natalie E. ; Orr, William E. ; Templeton, Justin P. ; Lu, Lu ; Williams, Robert ; Geisert, Eldon E. / Genetic pathways regulating glutamate levels in retinal müller cells. In: Neurochemical Research. 2011 ; Vol. 36, No. 4. pp. 594-603.
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