Genetic regulatory network analysis for Rpe65 in the eye of BXD mice

Hong Lu, Huaijin Guan, Hui Chen, Lu Lu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Previous studies have revealed that the mutation of Rpe65 plays a critical role in inherited retinal dystrophies. However, little is known about the genetic regulatory network for Rpe65 and inherited retinal dystrophies. We combined gene expression microarray analysis and quantitative trait loci (QTL) mapping to characterize the genetic regulatory network for Rpe65 expression in the eye of BXD recombinant inbred (RI) mice. Our analysis found that the expression level of Rpe65 exhibited much variation in the eye across the BXD RI strains and between the parental strains, C57BL/6J and DBA/2J. Expression QTL (eQTL) mapping showed that one microarray probe set of Rpe65 has highly significant linkage (Likelihood Ratio Statistic) scores. Moreover, the QTL was mapped to within 3 Mb of the location of the gene itself (Rpe65) as a cis-acting QTL. Through mapping the joint modulation of Rpe65, we identified Ches1/Foxn3 as downstream gene of Rpe65. Then the gene co-regulatory network analysis was constructed. The genetic genomics approach demonstrates the importance and the potential power of the eQTL studies in identifying genetic regulatory networks that contribute to inherited retinal dystrophies.

Original languageEnglish (US)
Title of host publicationAdvanced Research on Material Engineering, Chemistry, Bioinformatics, MECB 2011
Pages248-252
Number of pages5
DOIs
StatePublished - Jul 25 2011
Externally publishedYes
Event2011 International Conference on Material Engineering, Chemistry, Bioinformatics, MECB2011 - Wuhan, China
Duration: Aug 21 2011Aug 22 2011

Publication series

NameAdvanced Materials Research
Volume282-283
ISSN (Print)1022-6680

Other

Other2011 International Conference on Material Engineering, Chemistry, Bioinformatics, MECB2011
CountryChina
CityWuhan
Period8/21/118/22/11

Fingerprint

Electric network analysis
Genes
Microarrays
Gene expression
Modulation
Statistics

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Lu, H., Guan, H., Chen, H., & Lu, L. (2011). Genetic regulatory network analysis for Rpe65 in the eye of BXD mice. In Advanced Research on Material Engineering, Chemistry, Bioinformatics, MECB 2011 (pp. 248-252). (Advanced Materials Research; Vol. 282-283). https://doi.org/10.4028/www.scientific.net/AMR.282-283.248

Genetic regulatory network analysis for Rpe65 in the eye of BXD mice. / Lu, Hong; Guan, Huaijin; Chen, Hui; Lu, Lu.

Advanced Research on Material Engineering, Chemistry, Bioinformatics, MECB 2011. 2011. p. 248-252 (Advanced Materials Research; Vol. 282-283).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lu, H, Guan, H, Chen, H & Lu, L 2011, Genetic regulatory network analysis for Rpe65 in the eye of BXD mice. in Advanced Research on Material Engineering, Chemistry, Bioinformatics, MECB 2011. Advanced Materials Research, vol. 282-283, pp. 248-252, 2011 International Conference on Material Engineering, Chemistry, Bioinformatics, MECB2011, Wuhan, China, 8/21/11. https://doi.org/10.4028/www.scientific.net/AMR.282-283.248
Lu H, Guan H, Chen H, Lu L. Genetic regulatory network analysis for Rpe65 in the eye of BXD mice. In Advanced Research on Material Engineering, Chemistry, Bioinformatics, MECB 2011. 2011. p. 248-252. (Advanced Materials Research). https://doi.org/10.4028/www.scientific.net/AMR.282-283.248
Lu, Hong ; Guan, Huaijin ; Chen, Hui ; Lu, Lu. / Genetic regulatory network analysis for Rpe65 in the eye of BXD mice. Advanced Research on Material Engineering, Chemistry, Bioinformatics, MECB 2011. 2011. pp. 248-252 (Advanced Materials Research).
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