Genetic structure of the LXS panel of recombinant inbred mouse strains

A powerful resources for complex trait analysis

Robert Williams, Beth Bennett, Lu Lu, Jing Gu, John C. DeFries, Phyllis J. Carosone-Link, Brad A. Rikke, John K. Belknap, Thomas E. Johnson

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

The set of LXS recombinant inbred (RI) strains is a new and exceptionally large mapping panel that is suitable for the analysis of complex traits with comparatively high power. This panel consists of 77 strains - more than twice the size of other RI sets - and will typically provide sufficient statistical power (β = 0.8) to map quantitative trait loci (QTLs) that account for ∼25% of genetic variance with a genomewide p < 0.05. To charactrizethe genetic architecture of this new set of RI strains, we genotyped 330 MIT microsatellite markers distributed on all autosomes and the X Chromosome and assembled error-checked meiotic recombination maps that have an average F 2-adjusted marker spacing of ∼4 cM. The LXS panel has a genetic structure consistent with random segregation and subsequent fixation of alleles, the expected 3-4 x map expansion, a low level of nonsyntenic association among loci, and complete independence among all 77 strains. Although the parental inbred strains-Inbred Long-Sleep (ILS) and Inbred Short-Sleep (ISS) - were derived originally by selection from an 8-way heterogeneous stock selected for differential sensitivity to sedative effects of ethanol, the LXS panel is also segregating for many other traits. Thus, the LXS panel provides a powerful new resource for mapping complex traits across many systems and disciplines and should prove to be of great utility in modeling the genetics of complex diseases in human populations.

Original languageEnglish (US)
Pages (from-to)637-647
Number of pages11
JournalMammalian Genome
Volume15
Issue number8
DOIs
StatePublished - Aug 1 2004

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Inbred Strains Mice
Genetic Structures
Sleep
Inborn Genetic Diseases
Quantitative Trait Loci
X Chromosome
Hypnotics and Sedatives
Microsatellite Repeats
Genetic Recombination
Ethanol
Alleles
Population
Power (Psychology)

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Genetic structure of the LXS panel of recombinant inbred mouse strains : A powerful resources for complex trait analysis. / Williams, Robert; Bennett, Beth; Lu, Lu; Gu, Jing; DeFries, John C.; Carosone-Link, Phyllis J.; Rikke, Brad A.; Belknap, John K.; Johnson, Thomas E.

In: Mammalian Genome, Vol. 15, No. 8, 01.08.2004, p. 637-647.

Research output: Contribution to journalArticle

Williams, R, Bennett, B, Lu, L, Gu, J, DeFries, JC, Carosone-Link, PJ, Rikke, BA, Belknap, JK & Johnson, TE 2004, 'Genetic structure of the LXS panel of recombinant inbred mouse strains: A powerful resources for complex trait analysis', Mammalian Genome, vol. 15, no. 8, pp. 637-647. https://doi.org/10.1007/s00335-004-2380-6
Williams, Robert ; Bennett, Beth ; Lu, Lu ; Gu, Jing ; DeFries, John C. ; Carosone-Link, Phyllis J. ; Rikke, Brad A. ; Belknap, John K. ; Johnson, Thomas E. / Genetic structure of the LXS panel of recombinant inbred mouse strains : A powerful resources for complex trait analysis. In: Mammalian Genome. 2004 ; Vol. 15, No. 8. pp. 637-647.
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