Genetic variants of pre-microRNAs A499G(rs3746444) and T-196a2C(rsll614913) with ulcerative colitis (UC) and investigated with thiopurine-s-methyltransferase (TPMT) activity

Farideh Ghobadi, Asad Vaisi-Raygani, Fariborz Bahrehmand, Maryam Tanhapour, Amir Kiani, Zohreh Rahimi, Tayebeh Pourmotabbed

Research output: Contribution to journalArticle

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Abstract

Background: Abnormal expression and different splicing of miRNAs are involved in several human inflammatory disorders. It has been suggested that gene variants of miRNAs may be associated with increased risk of ulcerative colitis (UC). We aimed to evaluate the association of two SNPs (miRNA-A499G(rs3746444) and miRNA-T-192a96a2C(rsl 1614913)) with the risk of UC and monitor their effect on thiopurine-S-methyltransferase (TPMT) activity in Kurdish population of Iran. Methods: This case-control study was performed on 210 UC patients and 212 healthy individuals. Genotyping assay was performed using PCR-RFLP and the TPMT-activity was measured via non-extraction-HPLC method. Results: We found that the existence of GG genotypes and G allele of miRNA-A499G SNPs significantly increased the risk of UC by 1.76 and 1.32 times, respectively. The distribution of GG genotype (23.8% vs. 16%, χ 2 = 4.2, p = 0.041) and G allele (46.4% vs. 39.4%, = 4, p = 0.046) of miRNA-A499G, were significantly higher in UC patients compared to control group. Our results indicate that miRNA SNPs (miRNA-T-192a96a2C and miRNA-A499G) have no significant effect on TPMT activity of studied population. Conclusions: Our results, for the first time, demonstrate that the GG genotype and G allele of miRNA-A499G significantly increase the risk of UC. However, miRNA SNPs showed no significant effect on TPMT activity in studied population.

Original languageEnglish (US)
Pages (from-to)1683-1690
Number of pages8
JournalClinical Laboratory
Volume63
Issue number10
DOIs
StatePublished - Jan 1 2017

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thiopurine methyltransferase
Methyltransferases
MicroRNAs
Ulcerative Colitis
Single Nucleotide Polymorphism
Alleles
Genotype
Population

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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Genetic variants of pre-microRNAs A499G(rs3746444) and T-196a2C(rsll614913) with ulcerative colitis (UC) and investigated with thiopurine-s-methyltransferase (TPMT) activity. / Ghobadi, Farideh; Vaisi-Raygani, Asad; Bahrehmand, Fariborz; Tanhapour, Maryam; Kiani, Amir; Rahimi, Zohreh; Pourmotabbed, Tayebeh.

In: Clinical Laboratory, Vol. 63, No. 10, 01.01.2017, p. 1683-1690.

Research output: Contribution to journalArticle

Ghobadi, Farideh ; Vaisi-Raygani, Asad ; Bahrehmand, Fariborz ; Tanhapour, Maryam ; Kiani, Amir ; Rahimi, Zohreh ; Pourmotabbed, Tayebeh. / Genetic variants of pre-microRNAs A499G(rs3746444) and T-196a2C(rsll614913) with ulcerative colitis (UC) and investigated with thiopurine-s-methyltransferase (TPMT) activity. In: Clinical Laboratory. 2017 ; Vol. 63, No. 10. pp. 1683-1690.
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abstract = "Background: Abnormal expression and different splicing of miRNAs are involved in several human inflammatory disorders. It has been suggested that gene variants of miRNAs may be associated with increased risk of ulcerative colitis (UC). We aimed to evaluate the association of two SNPs (miRNA-A499G(rs3746444) and miRNA-T-192a96a2C(rsl 1614913)) with the risk of UC and monitor their effect on thiopurine-S-methyltransferase (TPMT) activity in Kurdish population of Iran. Methods: This case-control study was performed on 210 UC patients and 212 healthy individuals. Genotyping assay was performed using PCR-RFLP and the TPMT-activity was measured via non-extraction-HPLC method. Results: We found that the existence of GG genotypes and G allele of miRNA-A499G SNPs significantly increased the risk of UC by 1.76 and 1.32 times, respectively. The distribution of GG genotype (23.8{\%} vs. 16{\%}, χ 2 = 4.2, p = 0.041) and G allele (46.4{\%} vs. 39.4{\%}, = 4, p = 0.046) of miRNA-A499G, were significantly higher in UC patients compared to control group. Our results indicate that miRNA SNPs (miRNA-T-192a96a2C and miRNA-A499G) have no significant effect on TPMT activity of studied population. Conclusions: Our results, for the first time, demonstrate that the GG genotype and G allele of miRNA-A499G significantly increase the risk of UC. However, miRNA SNPs showed no significant effect on TPMT activity in studied population.",
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T1 - Genetic variants of pre-microRNAs A499G(rs3746444) and T-196a2C(rsll614913) with ulcerative colitis (UC) and investigated with thiopurine-s-methyltransferase (TPMT) activity

AU - Ghobadi, Farideh

AU - Vaisi-Raygani, Asad

AU - Bahrehmand, Fariborz

AU - Tanhapour, Maryam

AU - Kiani, Amir

AU - Rahimi, Zohreh

AU - Pourmotabbed, Tayebeh

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Background: Abnormal expression and different splicing of miRNAs are involved in several human inflammatory disorders. It has been suggested that gene variants of miRNAs may be associated with increased risk of ulcerative colitis (UC). We aimed to evaluate the association of two SNPs (miRNA-A499G(rs3746444) and miRNA-T-192a96a2C(rsl 1614913)) with the risk of UC and monitor their effect on thiopurine-S-methyltransferase (TPMT) activity in Kurdish population of Iran. Methods: This case-control study was performed on 210 UC patients and 212 healthy individuals. Genotyping assay was performed using PCR-RFLP and the TPMT-activity was measured via non-extraction-HPLC method. Results: We found that the existence of GG genotypes and G allele of miRNA-A499G SNPs significantly increased the risk of UC by 1.76 and 1.32 times, respectively. The distribution of GG genotype (23.8% vs. 16%, χ 2 = 4.2, p = 0.041) and G allele (46.4% vs. 39.4%, = 4, p = 0.046) of miRNA-A499G, were significantly higher in UC patients compared to control group. Our results indicate that miRNA SNPs (miRNA-T-192a96a2C and miRNA-A499G) have no significant effect on TPMT activity of studied population. Conclusions: Our results, for the first time, demonstrate that the GG genotype and G allele of miRNA-A499G significantly increase the risk of UC. However, miRNA SNPs showed no significant effect on TPMT activity in studied population.

AB - Background: Abnormal expression and different splicing of miRNAs are involved in several human inflammatory disorders. It has been suggested that gene variants of miRNAs may be associated with increased risk of ulcerative colitis (UC). We aimed to evaluate the association of two SNPs (miRNA-A499G(rs3746444) and miRNA-T-192a96a2C(rsl 1614913)) with the risk of UC and monitor their effect on thiopurine-S-methyltransferase (TPMT) activity in Kurdish population of Iran. Methods: This case-control study was performed on 210 UC patients and 212 healthy individuals. Genotyping assay was performed using PCR-RFLP and the TPMT-activity was measured via non-extraction-HPLC method. Results: We found that the existence of GG genotypes and G allele of miRNA-A499G SNPs significantly increased the risk of UC by 1.76 and 1.32 times, respectively. The distribution of GG genotype (23.8% vs. 16%, χ 2 = 4.2, p = 0.041) and G allele (46.4% vs. 39.4%, = 4, p = 0.046) of miRNA-A499G, were significantly higher in UC patients compared to control group. Our results indicate that miRNA SNPs (miRNA-T-192a96a2C and miRNA-A499G) have no significant effect on TPMT activity of studied population. Conclusions: Our results, for the first time, demonstrate that the GG genotype and G allele of miRNA-A499G significantly increase the risk of UC. However, miRNA SNPs showed no significant effect on TPMT activity in studied population.

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