Genome-wide gene expression profiles in antioxidant pathways and their potential sex differences and connections to Vitamin C in mice

Yan Jiao, Hong Chen, Jian Yan, Lishi Wang, Yue Huang, Xiaoyun Liu, Robert Williams, Lu Lu, Yongjun Wang, Weikuan Gu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Vitamin C (VC) is well known as an antioxidant in humans, primates and guinea pigs. Studies have suggested gender differences in VC requirements in humans, and gender differences in oxidant injury vulnerability in early life may represent a biological mechanism contributing to gender disparity in later life. Using spontaneous bone fracture (sfx) mice, which lack the gene for L-Gulonolactone oxidase (Gulo), we studied the potential sex difference in expression profiles of oxidative genes at the whole-genome level. Then, we analyzed data of gene expressions in a mouse population of recombinant inbred (RI) strains originally derived by crossing C57BL/6J (B6) and DBA/2J (D2) mice. Our data indicated that there were sex differences in the regulation of pre- and pro-oxidative genes in sfx mice. The associations of expression levels among Gulo, its partner genes and oxidative genes in the BXD (B6 × D2) RI strains showed a sex difference. Transcriptome mapping suggests that Gulo was regulated differently between female and male mice in BXD RI strains. Our study indicates the importance of investigating sex differences in Gulo and its oxidative function by using available mouse models.

Original languageEnglish (US)
Pages (from-to)10042-10062
Number of pages21
JournalInternational journal of molecular sciences
Volume14
Issue number5
DOIs
StatePublished - May 1 2013

Fingerprint

genome
ascorbic acid
gene expression
Vitamins
antioxidants
Antioxidants
Transcriptome
Gene expression
Sex Characteristics
Ascorbic Acid
mice
genes
Genes
oxidase
Genome
profiles
Oxidoreductases
swine
L-Gulonolactone Oxidase
Inbred DBA Mouse

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Genome-wide gene expression profiles in antioxidant pathways and their potential sex differences and connections to Vitamin C in mice. / Jiao, Yan; Chen, Hong; Yan, Jian; Wang, Lishi; Huang, Yue; Liu, Xiaoyun; Williams, Robert; Lu, Lu; Wang, Yongjun; Gu, Weikuan.

In: International journal of molecular sciences, Vol. 14, No. 5, 01.05.2013, p. 10042-10062.

Research output: Contribution to journalArticle

@article{2c69eddfc66e4b1eb13e2581cce2a5df,
title = "Genome-wide gene expression profiles in antioxidant pathways and their potential sex differences and connections to Vitamin C in mice",
abstract = "Vitamin C (VC) is well known as an antioxidant in humans, primates and guinea pigs. Studies have suggested gender differences in VC requirements in humans, and gender differences in oxidant injury vulnerability in early life may represent a biological mechanism contributing to gender disparity in later life. Using spontaneous bone fracture (sfx) mice, which lack the gene for L-Gulonolactone oxidase (Gulo), we studied the potential sex difference in expression profiles of oxidative genes at the whole-genome level. Then, we analyzed data of gene expressions in a mouse population of recombinant inbred (RI) strains originally derived by crossing C57BL/6J (B6) and DBA/2J (D2) mice. Our data indicated that there were sex differences in the regulation of pre- and pro-oxidative genes in sfx mice. The associations of expression levels among Gulo, its partner genes and oxidative genes in the BXD (B6 × D2) RI strains showed a sex difference. Transcriptome mapping suggests that Gulo was regulated differently between female and male mice in BXD RI strains. Our study indicates the importance of investigating sex differences in Gulo and its oxidative function by using available mouse models.",
author = "Yan Jiao and Hong Chen and Jian Yan and Lishi Wang and Yue Huang and Xiaoyun Liu and Robert Williams and Lu Lu and Yongjun Wang and Weikuan Gu",
year = "2013",
month = "5",
day = "1",
doi = "10.3390/ijms140510042",
language = "English (US)",
volume = "14",
pages = "10042--10062",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "5",

}

TY - JOUR

T1 - Genome-wide gene expression profiles in antioxidant pathways and their potential sex differences and connections to Vitamin C in mice

AU - Jiao, Yan

AU - Chen, Hong

AU - Yan, Jian

AU - Wang, Lishi

AU - Huang, Yue

AU - Liu, Xiaoyun

AU - Williams, Robert

AU - Lu, Lu

AU - Wang, Yongjun

AU - Gu, Weikuan

PY - 2013/5/1

Y1 - 2013/5/1

N2 - Vitamin C (VC) is well known as an antioxidant in humans, primates and guinea pigs. Studies have suggested gender differences in VC requirements in humans, and gender differences in oxidant injury vulnerability in early life may represent a biological mechanism contributing to gender disparity in later life. Using spontaneous bone fracture (sfx) mice, which lack the gene for L-Gulonolactone oxidase (Gulo), we studied the potential sex difference in expression profiles of oxidative genes at the whole-genome level. Then, we analyzed data of gene expressions in a mouse population of recombinant inbred (RI) strains originally derived by crossing C57BL/6J (B6) and DBA/2J (D2) mice. Our data indicated that there were sex differences in the regulation of pre- and pro-oxidative genes in sfx mice. The associations of expression levels among Gulo, its partner genes and oxidative genes in the BXD (B6 × D2) RI strains showed a sex difference. Transcriptome mapping suggests that Gulo was regulated differently between female and male mice in BXD RI strains. Our study indicates the importance of investigating sex differences in Gulo and its oxidative function by using available mouse models.

AB - Vitamin C (VC) is well known as an antioxidant in humans, primates and guinea pigs. Studies have suggested gender differences in VC requirements in humans, and gender differences in oxidant injury vulnerability in early life may represent a biological mechanism contributing to gender disparity in later life. Using spontaneous bone fracture (sfx) mice, which lack the gene for L-Gulonolactone oxidase (Gulo), we studied the potential sex difference in expression profiles of oxidative genes at the whole-genome level. Then, we analyzed data of gene expressions in a mouse population of recombinant inbred (RI) strains originally derived by crossing C57BL/6J (B6) and DBA/2J (D2) mice. Our data indicated that there were sex differences in the regulation of pre- and pro-oxidative genes in sfx mice. The associations of expression levels among Gulo, its partner genes and oxidative genes in the BXD (B6 × D2) RI strains showed a sex difference. Transcriptome mapping suggests that Gulo was regulated differently between female and male mice in BXD RI strains. Our study indicates the importance of investigating sex differences in Gulo and its oxidative function by using available mouse models.

UR - http://www.scopus.com/inward/record.url?scp=84877817928&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877817928&partnerID=8YFLogxK

U2 - 10.3390/ijms140510042

DO - 10.3390/ijms140510042

M3 - Article

VL - 14

SP - 10042

EP - 10062

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 5

ER -