Genome-wide transcriptional analysis in S. cerevisiae by mini-array membrane hybridization

Kathleen H. Cox, Anna B. Pinchak, Terrance Cooper

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Access to the powerful micro-array analytical methods used for genome-wide transcriptional analysis has so far been restricted by the high cost and/or lack of availability of the sophisticated instrumentation and materials needed to perform it. Mini-array membrane hybridization provides a less expensive alternative. The reliability of this technique, however, is not well documented and its reported use has, up to this point, been very limited. Our objective was to test whether or not mini-array membrane hybridization would reliably identify genes whose expression was controlled by a specific set of genetic and/or physiological signals. Our results demonstrate that mini-array hybridization can correctly identify genes whose expression is known to be controlled by the GATA-factor regulatory network in S. cerevisiae and in addition can reliably identify genes not previously reported to be associated with this nitrogen control system.

Original languageEnglish (US)
Pages (from-to)703-713
Number of pages11
JournalYeast
Volume15
Issue number8
DOIs
StatePublished - Jun 15 1999

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Gene expression
Saccharomyces cerevisiae
Genes
GATA Transcription Factors
Genome
Membranes
Gene Expression
Nitrogen
Availability
Control systems
Costs and Cost Analysis
Costs

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Genome-wide transcriptional analysis in S. cerevisiae by mini-array membrane hybridization. / Cox, Kathleen H.; Pinchak, Anna B.; Cooper, Terrance.

In: Yeast, Vol. 15, No. 8, 15.06.1999, p. 703-713.

Research output: Contribution to journalArticle

Cox, Kathleen H. ; Pinchak, Anna B. ; Cooper, Terrance. / Genome-wide transcriptional analysis in S. cerevisiae by mini-array membrane hybridization. In: Yeast. 1999 ; Vol. 15, No. 8. pp. 703-713.
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