Genomic analysis of detoxification genes in the mosquito Aedes aegypti

Clare Strode, Charles S. Wondji, Jean Philippe David, Nicola J. Hawkes, Nongkran Lumjuan, David Nelson, David R. Drane, S. H.P.Parakrama Karunaratne, Janet Hemingway, William C. Black IV, Hilary Ranson

Research output: Contribution to journalArticle

210 Citations (Scopus)

Abstract

Annotation of the recently determined genome sequence of the major dengue vector, Aedes aegypti, reveals an abundance of detoxification genes. Here, we report the presence of 235 members of the cytochrome P450, glutathione transferase and carboxy/cholinesterase families in Ae. aegypti. This gene count represents an increase of 58% and 36% compared with the fruitfly, Drosophila melanogaster, and the malaria mosquito, Anopheles gambiae, respectively. The expansion is not uniform within the gene families. Secure orthologs can be found across the insect species for enzymes that have presumed or proven biosynthetic or housekeeping roles. In contrast, subsets of these gene families that are associated with general xenobiotic detoxification, in particular the CYP6, CYP9 and alpha esterase families, have expanded in Ae. aegypti. In order to identify detoxification genes associated with resistance to insecticides we constructed an array containing unique oligonucleotide probes for these genes and compared their expression level in insecticide resistant and susceptible strains. Several candidate genes were identified with the majority belonging to two gene families, the CYP9 P450s and the Epsilon GSTs. This 'Ae. aegypti Detox Chip' will facilitate the implementation of insecticide resistance management strategies for arboviral control programmes.

Original languageEnglish (US)
Pages (from-to)113-123
Number of pages11
JournalInsect Biochemistry and Molecular Biology
Volume38
Issue number1
DOIs
StatePublished - Jan 1 2008

Fingerprint

Detoxification
Aedes
Aedes aegypti
Culicidae
Genes
genomics
genes
Insecticide Resistance
Insecticides
insecticides
Anopheles gambiae
Housekeeping
oligonucleotide probes
resistance management
dengue
Dengue
Oligonucleotide Probes
cholinesterase
insecticide resistance
Cholinesterases

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Insect Science

Cite this

Strode, C., Wondji, C. S., David, J. P., Hawkes, N. J., Lumjuan, N., Nelson, D., ... Ranson, H. (2008). Genomic analysis of detoxification genes in the mosquito Aedes aegypti. Insect Biochemistry and Molecular Biology, 38(1), 113-123. https://doi.org/10.1016/j.ibmb.2007.09.007

Genomic analysis of detoxification genes in the mosquito Aedes aegypti. / Strode, Clare; Wondji, Charles S.; David, Jean Philippe; Hawkes, Nicola J.; Lumjuan, Nongkran; Nelson, David; Drane, David R.; Karunaratne, S. H.P.Parakrama; Hemingway, Janet; Black IV, William C.; Ranson, Hilary.

In: Insect Biochemistry and Molecular Biology, Vol. 38, No. 1, 01.01.2008, p. 113-123.

Research output: Contribution to journalArticle

Strode, C, Wondji, CS, David, JP, Hawkes, NJ, Lumjuan, N, Nelson, D, Drane, DR, Karunaratne, SHPP, Hemingway, J, Black IV, WC & Ranson, H 2008, 'Genomic analysis of detoxification genes in the mosquito Aedes aegypti', Insect Biochemistry and Molecular Biology, vol. 38, no. 1, pp. 113-123. https://doi.org/10.1016/j.ibmb.2007.09.007
Strode, Clare ; Wondji, Charles S. ; David, Jean Philippe ; Hawkes, Nicola J. ; Lumjuan, Nongkran ; Nelson, David ; Drane, David R. ; Karunaratne, S. H.P.Parakrama ; Hemingway, Janet ; Black IV, William C. ; Ranson, Hilary. / Genomic analysis of detoxification genes in the mosquito Aedes aegypti. In: Insect Biochemistry and Molecular Biology. 2008 ; Vol. 38, No. 1. pp. 113-123.
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