Genomic analysis of variation in hindlimb musculature of mice fromthe C57BL/6J and DBA/2J Lineage

Arimantas Lionikas, Örjan Carlborg, Lu Lu, Jeremy L. Peirce, Robert Williams, Fushun Yu, George P. Vogler, Gerald E. McClearn, David A. Blizard

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The precise locations of attachment points of muscle to bone - the origin and insertion sites - are crucial anatomical and functional characteristics that influence locomotor performance. Mechanisms that control the development of these interactions between muscle, tendon, and bone are currently not well understood. In a subset of BXD recombinant inbred (RI) strains derived from the C57BL/6J and DBA/2J strains, we observed a soleus femoral attachment anomaly (SFAA) that was rare in both parental strains (Lionikas, Glover et al. 2006). The aim of the present study was to assess suitability of SFAA as a model to study the genetic mechanisms underlying variation in musculoskeletal anatomy. We scored the incidence of SFAA in 55 BXD strains (n = 9 to 136, median = 26, phenotyped animals per strain, for a total number of 2367). Seven strains (BXD1, 12, 38, 43, 48, 54, and 56) exhibited a high incidence of unilateral SFAA (47-89%), whereas 23 strains scored 0%. Exploration of the mechanisms underlying SFAA in 2 high incidence strains, BXD1 and BXD38, indicated that SFAA-relevant genes are to be found in both C57BL/6J and DBA/2J regions of the BXD1 genome. However, not all alleles relevant for the expression of the phenotype were shared between the 2 high-incidence BXD strains. In conclusion, the anatomical origin of the soleus muscle in mouse is controlled by a polygenic system. A panel of BXD RI strains is a useful tool in exploring the genetic mechanisms underlying SFAA and improving our understanding of musculoskeletal development.

Original languageEnglish (US)
Pages (from-to)360-367
Number of pages8
JournalJournal of Heredity
Volume101
Issue number3
DOIs
StatePublished - May 6 2010

Fingerprint

Hindlimb
Thigh
Inbred C57BL Mouse
Incidence
Musculoskeletal Development
Bone and Bones
Muscles
Tendons
Anatomy
Skeletal Muscle
Alleles
Genome
Phenotype
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Genomic analysis of variation in hindlimb musculature of mice fromthe C57BL/6J and DBA/2J Lineage. / Lionikas, Arimantas; Carlborg, Örjan; Lu, Lu; Peirce, Jeremy L.; Williams, Robert; Yu, Fushun; Vogler, George P.; McClearn, Gerald E.; Blizard, David A.

In: Journal of Heredity, Vol. 101, No. 3, 06.05.2010, p. 360-367.

Research output: Contribution to journalArticle

Lionikas, A, Carlborg, Ö, Lu, L, Peirce, JL, Williams, R, Yu, F, Vogler, GP, McClearn, GE & Blizard, DA 2010, 'Genomic analysis of variation in hindlimb musculature of mice fromthe C57BL/6J and DBA/2J Lineage', Journal of Heredity, vol. 101, no. 3, pp. 360-367. https://doi.org/10.1093/jhered/esq023
Lionikas, Arimantas ; Carlborg, Örjan ; Lu, Lu ; Peirce, Jeremy L. ; Williams, Robert ; Yu, Fushun ; Vogler, George P. ; McClearn, Gerald E. ; Blizard, David A. / Genomic analysis of variation in hindlimb musculature of mice fromthe C57BL/6J and DBA/2J Lineage. In: Journal of Heredity. 2010 ; Vol. 101, No. 3. pp. 360-367.
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AU - Peirce, Jeremy L.

AU - Williams, Robert

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AU - Vogler, George P.

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