Genomic scanning for expressed sequences in Xp21 identifies the glycerol kinase gene

Weiwen Guo, Kim Worley, Volker Adams, Jestina Mason, Desirée Sylvester-Jackson, Yao Hua Zhang, Jeffrey Towbin, Donna D. Fogt, Stella Madu, David A. Wheeler, Edward R.B. McCabe

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Rapid genomic scanning methods are required to identify expressed sequences and we report an efficient, sensitive and specific approach which relies upon hybridization of an amplified, labeled cDNA library to digested cosmid DNA. We identified expressed sequences within a cosmid in the glycerol kinase (GK) “critical region” of Xp21 that had impressive similarity to prokaryotic GKs. We used this genomic sequence information to clone the human hepatic GK cDNA. Independent confirmation of the identity of this gene was obtained by functional complementation of GK deficient E. coli mutants with a construct containing the complete human X–linked GK coding sequence.

Original languageEnglish (US)
Pages (from-to)367-372
Number of pages6
JournalNature Genetics
Volume4
Issue number4
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

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Glycerol Kinase
Cosmids
Genes
Gene Library
Complementary DNA
Clone Cells
Escherichia coli
Liver
DNA

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Guo, W., Worley, K., Adams, V., Mason, J., Sylvester-Jackson, D., Zhang, Y. H., ... McCabe, E. R. B. (1993). Genomic scanning for expressed sequences in Xp21 identifies the glycerol kinase gene. Nature Genetics, 4(4), 367-372. https://doi.org/10.1038/ng0893-367

Genomic scanning for expressed sequences in Xp21 identifies the glycerol kinase gene. / Guo, Weiwen; Worley, Kim; Adams, Volker; Mason, Jestina; Sylvester-Jackson, Desirée; Zhang, Yao Hua; Towbin, Jeffrey; Fogt, Donna D.; Madu, Stella; Wheeler, David A.; McCabe, Edward R.B.

In: Nature Genetics, Vol. 4, No. 4, 01.01.1993, p. 367-372.

Research output: Contribution to journalArticle

Guo, W, Worley, K, Adams, V, Mason, J, Sylvester-Jackson, D, Zhang, YH, Towbin, J, Fogt, DD, Madu, S, Wheeler, DA & McCabe, ERB 1993, 'Genomic scanning for expressed sequences in Xp21 identifies the glycerol kinase gene', Nature Genetics, vol. 4, no. 4, pp. 367-372. https://doi.org/10.1038/ng0893-367
Guo W, Worley K, Adams V, Mason J, Sylvester-Jackson D, Zhang YH et al. Genomic scanning for expressed sequences in Xp21 identifies the glycerol kinase gene. Nature Genetics. 1993 Jan 1;4(4):367-372. https://doi.org/10.1038/ng0893-367
Guo, Weiwen ; Worley, Kim ; Adams, Volker ; Mason, Jestina ; Sylvester-Jackson, Desirée ; Zhang, Yao Hua ; Towbin, Jeffrey ; Fogt, Donna D. ; Madu, Stella ; Wheeler, David A. ; McCabe, Edward R.B. / Genomic scanning for expressed sequences in Xp21 identifies the glycerol kinase gene. In: Nature Genetics. 1993 ; Vol. 4, No. 4. pp. 367-372.
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