Glial elements contribute to stress-induced torsinA expression in the CNS and peripheral nervous system

Y. Zhao, Jianfeng Xiao, M. Ueda, Y. Wang, M. Hines, Thaddeus Nowak, Mark Ledoux

Research output: Contribution to journalArticle

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Abstract

DYT1 dystonia is caused by a single GAG deletion in exon 5 of TOR1A, the gene encoding torsinA, a putative chaperone protein. In this study, central and peripheral nervous system perturbations (transient forebrain ischemia and sciatic nerve transection, respectively) were used to examine the systems biology of torsinA in rats. After forebrain ischemia, quantitative real-time reverse transcriptase-polymerase chain reaction identified increased torsinA transcript levels in hippocampus, cerebral cortex, thalamus, striatum, and cerebellum at 24 h and 7 days. Expression declined toward sham values by 14 days in striatum, thalamus and cortex, and by 21 days in cerebellum and hippocampus. TorsinA transcripts were localized to dentate granule cells and pyramidal neurons in control hippocampus and were moderately elevated in these cell populations at 24 h after ischemia, after which CA1 expression was reduced, consistent with the loss of this vulnerable neuronal population. Increased in situ hybridization signal in CA1 stratum radiatum, stratum lacunosum-moleculare, and stratum oriens at 7 days after ischemia was correlated with the detection of torsinA immunoreactivity in interneurons and reactive astrocytes at 7 and 14 days. Sciatic nerve transection increased torsinA transcript levels between 24 h and 7 days in both ipsilateral and contralateral dorsal root ganglia (DRG). However, increased torsinA immunoreactivity was localized to both ganglion cells and satellite cells in ipsilateral DRG but was restricted to satellite cells contralaterally. These results suggest that torsinA participates in the response of neural tissue to central and peripheral insults and its sustained up-regulation indicates that torsinA may contribute to remodeling of neuronal circuitry. The striking induction of torsinA in astrocytes and satellite cells points to the potential involvement of glial elements in the pathobiology of DYT1 dystonia.

Original languageEnglish (US)
Pages (from-to)439-453
Number of pages15
JournalNeuroscience
Volume155
Issue number2
DOIs
StatePublished - Aug 13 2008

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Peripheral Nervous System
Neuroglia
Ischemia
Hippocampus
Dystonia
Spinal Ganglia
Sciatic Nerve
Prosencephalon
Thalamus
Astrocytes
Cerebellum
Hippocampal CA1 Region
Neuronal Plasticity
Systems Biology
Pyramidal Cells
Interneurons
Vulnerable Populations
Reverse Transcriptase Polymerase Chain Reaction
Ganglia
Cerebral Cortex

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Glial elements contribute to stress-induced torsinA expression in the CNS and peripheral nervous system. / Zhao, Y.; Xiao, Jianfeng; Ueda, M.; Wang, Y.; Hines, M.; Nowak, Thaddeus; Ledoux, Mark.

In: Neuroscience, Vol. 155, No. 2, 13.08.2008, p. 439-453.

Research output: Contribution to journalArticle

Zhao, Y. ; Xiao, Jianfeng ; Ueda, M. ; Wang, Y. ; Hines, M. ; Nowak, Thaddeus ; Ledoux, Mark. / Glial elements contribute to stress-induced torsinA expression in the CNS and peripheral nervous system. In: Neuroscience. 2008 ; Vol. 155, No. 2. pp. 439-453.
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