Gln3p Is capable of binding to UASNTR elements and activating transcription in Saccharomyces cerevisiae

Thomas S. Cunningham, Vladimir V. Svetlov, Rajendra Rai, William Smart, Terrance Cooper

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

When readily used nitrogen sources are available, the expression of genes encoding proteins needed to transport and metabolize poorly used nitrogen sources is repressed to low levels; this physiological response has been designated nitrogen catabolite repression (NCR). The cis-acting upstream activation sequence (UAS) element UASNTR mediates Gln3p-dependent, NCR-sensitive transcription and consists of two separated dodecanucleotides, each containing the core sequence GATAA. Gln3p, produced in Escherichia coli and hence free of all other yeast proteins, specifically binds to wild-type UASNTR sequences and DNA fragments derived from a variety of NCR-sensitive promoters (GDH2, CAR1, DAL3, PUT1, UGA4, and GLNI). A LexA-Gln3 fusion protein supported transcriptional activation when bound to one or more LexAp binding sites upstream of a minimal CYC1-derived promoter devoid of UAS elements. LexAp-Gln3p activation of transcription was largely independent of the nitrogen source used for growth. These data argue that Gln3p is capable of direct UASNTR binding and participates in transcriptional activation of NCR-sensitive genes.

Original languageEnglish (US)
Pages (from-to)3470-3479
Number of pages10
JournalJournal of bacteriology
Volume178
Issue number12
DOIs
StatePublished - Jan 1 1996

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Saccharomyces cerevisiae
Nitrogen
Catabolite Repression
Transcriptional Activation
Fungal Proteins
Proteins
Binding Sites
Escherichia coli
Growth
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Gln3p Is capable of binding to UASNTR elements and activating transcription in Saccharomyces cerevisiae. / Cunningham, Thomas S.; Svetlov, Vladimir V.; Rai, Rajendra; Smart, William; Cooper, Terrance.

In: Journal of bacteriology, Vol. 178, No. 12, 01.01.1996, p. 3470-3479.

Research output: Contribution to journalArticle

Cunningham, Thomas S. ; Svetlov, Vladimir V. ; Rai, Rajendra ; Smart, William ; Cooper, Terrance. / Gln3p Is capable of binding to UASNTR elements and activating transcription in Saccharomyces cerevisiae. In: Journal of bacteriology. 1996 ; Vol. 178, No. 12. pp. 3470-3479.
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