GLP-2 stimulates intestinal growth in premature TPN-fed pigs by suppressing proteolysis and apoptosis

D. G. Burrin, B. Stoll, R. Jiang, Y. Petersen, J. Elnif, Randal Buddington, M. Schmidt, J. J. Holst, B. Hartmann, P. T. Sangild

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

We wished to determine whether exogenous glucagon-like peptide (GLP)-2 infusion stimulates intestinal growth in parenterally fed immature pigs. Piglets (106-108 days gestation) were given parenteral nutrient infusion (TPN), TPN + human GLP-2 (25 nmol·kg-1·day-1>), or sow's milk enterally (ENT) for 6 days. Intestinal protein synthesis was then measured in vivo after a bolus dose of [1-13C]phenylalanine, and degradation was calculated from the difference between protein accretion and synthesis. Crypt cell proliferation and apoptosis were measured in situ by 5-bromodeoxyuridine (BrdU) and terminal dUTP nick-end labeling (TUNEL), respectively. Intestinal protein and DNA accretion rates and villus heights were similar in GLP-2 and ENT pigs, and both were higher (P < 0.05) than in TPN pigs. GLP-2 decreased fractional protein degradation rate, whereas ENT increased fractional protein synthesis rate compared with TPN pigs. Percentage of TUNEL-positive cells in GLP-2 and ENT groups was 48 and 64% lower, respectively, than in TPN group (P < 0.05). However, ENT, but not GLP-2, increased percentage of BrdU-positive crypt cells above that in TPN piglets. We conclude that GLP-2 increases intestinal growth in premature, TPN-fed pigs by decreasing proteolysis and apoptosis, whereas enteral nutrition acts via increased protein synthesis and cell proliferation and decreased apoptosis.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume279
Issue number6 42-6
StatePublished - Dec 28 2000
Externally publishedYes

Fingerprint

Glucagon-Like Peptide 2
Proteolysis
Swine
Apoptosis
Growth
Bromodeoxyuridine
Proteins
Cell Proliferation
Parenteral Infusions
Enteral Nutrition
Phenylalanine
Milk
Food
Pregnancy
DNA

All Science Journal Classification (ASJC) codes

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Cite this

GLP-2 stimulates intestinal growth in premature TPN-fed pigs by suppressing proteolysis and apoptosis. / Burrin, D. G.; Stoll, B.; Jiang, R.; Petersen, Y.; Elnif, J.; Buddington, Randal; Schmidt, M.; Holst, J. J.; Hartmann, B.; Sangild, P. T.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 279, No. 6 42-6, 28.12.2000.

Research output: Contribution to journalArticle

Burrin, DG, Stoll, B, Jiang, R, Petersen, Y, Elnif, J, Buddington, R, Schmidt, M, Holst, JJ, Hartmann, B & Sangild, PT 2000, 'GLP-2 stimulates intestinal growth in premature TPN-fed pigs by suppressing proteolysis and apoptosis', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 279, no. 6 42-6.
Burrin, D. G. ; Stoll, B. ; Jiang, R. ; Petersen, Y. ; Elnif, J. ; Buddington, Randal ; Schmidt, M. ; Holst, J. J. ; Hartmann, B. ; Sangild, P. T. / GLP-2 stimulates intestinal growth in premature TPN-fed pigs by suppressing proteolysis and apoptosis. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2000 ; Vol. 279, No. 6 42-6.
@article{c77c6b507135439889fce907d3440cda,
title = "GLP-2 stimulates intestinal growth in premature TPN-fed pigs by suppressing proteolysis and apoptosis",
abstract = "We wished to determine whether exogenous glucagon-like peptide (GLP)-2 infusion stimulates intestinal growth in parenterally fed immature pigs. Piglets (106-108 days gestation) were given parenteral nutrient infusion (TPN), TPN + human GLP-2 (25 nmol·kg-1·day-1>), or sow's milk enterally (ENT) for 6 days. Intestinal protein synthesis was then measured in vivo after a bolus dose of [1-13C]phenylalanine, and degradation was calculated from the difference between protein accretion and synthesis. Crypt cell proliferation and apoptosis were measured in situ by 5-bromodeoxyuridine (BrdU) and terminal dUTP nick-end labeling (TUNEL), respectively. Intestinal protein and DNA accretion rates and villus heights were similar in GLP-2 and ENT pigs, and both were higher (P < 0.05) than in TPN pigs. GLP-2 decreased fractional protein degradation rate, whereas ENT increased fractional protein synthesis rate compared with TPN pigs. Percentage of TUNEL-positive cells in GLP-2 and ENT groups was 48 and 64{\%} lower, respectively, than in TPN group (P < 0.05). However, ENT, but not GLP-2, increased percentage of BrdU-positive crypt cells above that in TPN piglets. We conclude that GLP-2 increases intestinal growth in premature, TPN-fed pigs by decreasing proteolysis and apoptosis, whereas enteral nutrition acts via increased protein synthesis and cell proliferation and decreased apoptosis.",
author = "Burrin, {D. G.} and B. Stoll and R. Jiang and Y. Petersen and J. Elnif and Randal Buddington and M. Schmidt and Holst, {J. J.} and B. Hartmann and Sangild, {P. T.}",
year = "2000",
month = "12",
day = "28",
language = "English (US)",
volume = "279",
journal = "American Journal of Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "6 42-6",

}

TY - JOUR

T1 - GLP-2 stimulates intestinal growth in premature TPN-fed pigs by suppressing proteolysis and apoptosis

AU - Burrin, D. G.

AU - Stoll, B.

AU - Jiang, R.

AU - Petersen, Y.

AU - Elnif, J.

AU - Buddington, Randal

AU - Schmidt, M.

AU - Holst, J. J.

AU - Hartmann, B.

AU - Sangild, P. T.

PY - 2000/12/28

Y1 - 2000/12/28

N2 - We wished to determine whether exogenous glucagon-like peptide (GLP)-2 infusion stimulates intestinal growth in parenterally fed immature pigs. Piglets (106-108 days gestation) were given parenteral nutrient infusion (TPN), TPN + human GLP-2 (25 nmol·kg-1·day-1>), or sow's milk enterally (ENT) for 6 days. Intestinal protein synthesis was then measured in vivo after a bolus dose of [1-13C]phenylalanine, and degradation was calculated from the difference between protein accretion and synthesis. Crypt cell proliferation and apoptosis were measured in situ by 5-bromodeoxyuridine (BrdU) and terminal dUTP nick-end labeling (TUNEL), respectively. Intestinal protein and DNA accretion rates and villus heights were similar in GLP-2 and ENT pigs, and both were higher (P < 0.05) than in TPN pigs. GLP-2 decreased fractional protein degradation rate, whereas ENT increased fractional protein synthesis rate compared with TPN pigs. Percentage of TUNEL-positive cells in GLP-2 and ENT groups was 48 and 64% lower, respectively, than in TPN group (P < 0.05). However, ENT, but not GLP-2, increased percentage of BrdU-positive crypt cells above that in TPN piglets. We conclude that GLP-2 increases intestinal growth in premature, TPN-fed pigs by decreasing proteolysis and apoptosis, whereas enteral nutrition acts via increased protein synthesis and cell proliferation and decreased apoptosis.

AB - We wished to determine whether exogenous glucagon-like peptide (GLP)-2 infusion stimulates intestinal growth in parenterally fed immature pigs. Piglets (106-108 days gestation) were given parenteral nutrient infusion (TPN), TPN + human GLP-2 (25 nmol·kg-1·day-1>), or sow's milk enterally (ENT) for 6 days. Intestinal protein synthesis was then measured in vivo after a bolus dose of [1-13C]phenylalanine, and degradation was calculated from the difference between protein accretion and synthesis. Crypt cell proliferation and apoptosis were measured in situ by 5-bromodeoxyuridine (BrdU) and terminal dUTP nick-end labeling (TUNEL), respectively. Intestinal protein and DNA accretion rates and villus heights were similar in GLP-2 and ENT pigs, and both were higher (P < 0.05) than in TPN pigs. GLP-2 decreased fractional protein degradation rate, whereas ENT increased fractional protein synthesis rate compared with TPN pigs. Percentage of TUNEL-positive cells in GLP-2 and ENT groups was 48 and 64% lower, respectively, than in TPN group (P < 0.05). However, ENT, but not GLP-2, increased percentage of BrdU-positive crypt cells above that in TPN piglets. We conclude that GLP-2 increases intestinal growth in premature, TPN-fed pigs by decreasing proteolysis and apoptosis, whereas enteral nutrition acts via increased protein synthesis and cell proliferation and decreased apoptosis.

UR - http://www.scopus.com/inward/record.url?scp=0033638570&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033638570&partnerID=8YFLogxK

M3 - Article

C2 - 11093948

AN - SCOPUS:0033638570

VL - 279

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 1931-857X

IS - 6 42-6

ER -