Glucagon-like peptide 2 has limited efficacy to increase nutrient absorption in fetal and preterm pigs

Per T. Sangild, Christiane Malo, Mette Schmidt, Yvette M. Petersen, Jan Elnif, Jens J. Holst, Randal Buddington

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Exogenous glucagon-like peptide 2 (GLP-2) prevents intestinal atrophy and increases nutrient absorption in term newborn pigs receiving total parenteral nutrition (TPN). We tested the hypothesis that the immature intestine of fetuses and preterm neonates has a diminished nutrient absorption response to exogenous GLP-2. This was accomplished using catheterized fetal pigs infused for 6 days (87-91% of gestation) with GLP-2 (25 nmol·kg-1·day -1 iv; n = 7) or saline (n = 7), and cesarean-delivered preterm pigs (92% of gestation) that received TPN with GLP-2 (25 nmol·kg -1·day-1 iv; n = 8) or saline (n = 7) for 6 days after birth. Responses to GLP-2 were assessed by measuring intestinal dimensions, absorption of nutrients (glucose, leucine, lysine, proline) by intact tissues and brush border membrane vesicles, and abundance of sodium-glucose cotransporter mRNA. Infusion of GLP-2 increased circulating GLP-2 levels in fetuses, but did not increase intestinal mass or absorption of nutrients by intact tissues and brush border membrane vesicles, except for lysine. Administration of exogenous GLP-2 to preterm TPN-fed pigs similarly did not increase rates of nutrient absorption, yet nutrient absorption capacities of the entire small intestine tended to increase (+10-20%, P < 0.10) compared with TPN alone due to increased intestinal mass (+30%, P < 0.05). GLP-2 infusion did not increase sodium-glucose cotransporter-1 mRNA abundance in fetuses or postnatal preterm pigs. Hence, the efficacy of exogenous GLP-2 to improve nutrient absorption by the intestine of fetal and preterm pigs is limited compared with term pigs and more mature animals and humans.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume293
Issue number6
DOIs
StatePublished - Dec 1 2007
Externally publishedYes

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Glucagon-Like Peptide 2
Swine
Food
Total Parenteral Nutrition
Fetus
Microvilli
Lysine
Intestines
Sodium-Glucose Transporter 1
Sodium-Glucose Transport Proteins
Pregnancy
Messenger RNA
Membranes
Intestinal Absorption
Proline
Leucine
Small Intestine
Atrophy

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Glucagon-like peptide 2 has limited efficacy to increase nutrient absorption in fetal and preterm pigs. / Sangild, Per T.; Malo, Christiane; Schmidt, Mette; Petersen, Yvette M.; Elnif, Jan; Holst, Jens J.; Buddington, Randal.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 293, No. 6, 01.12.2007.

Research output: Contribution to journalArticle

Sangild, Per T. ; Malo, Christiane ; Schmidt, Mette ; Petersen, Yvette M. ; Elnif, Jan ; Holst, Jens J. ; Buddington, Randal. / Glucagon-like peptide 2 has limited efficacy to increase nutrient absorption in fetal and preterm pigs. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2007 ; Vol. 293, No. 6.
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