Glucagon-like peptide-2 protects against TPN-induced intestinal hexose malabsorption in enterally refed piglets

J. J. Cottrell, B. Stoll, Randal Buddington, J. E. Stephens, L. Cui, X. Chang, D. G. Burrin

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Premature infants receiving chronic total parenteral nutrition (TPN) due to feeding intolerance develop intestinal atrophy and reduced nutrient absorption. Although providing the intestinal trophic hormone glucagon-like peptide-2 (GLP-2) during chronic TPN improves intestinal growth and morphology, it is uncertain whether GLP-2 enhances absorptive function. We placed catheters in the carotid artery, jugular and portal veins, duodenum, and a portal vein flow probe in piglets before providing either enteral formula (ENT), TPN or a coinfusion of TPN plus GLP-2 for 6 days. On postoperative day 7, all piglets were fed enterally and digestive functions were evaluated in vivo using dual infusion of enteral ( 13C) and intravenous ( 2H) glucose, in vitro by measuring mucosal lactase activity and rates of apical glucose transport, and by assessing the abundances of sodium glucose transporter-1 (SGLT-1) and glucose transporter-2 (GLUT2). Both ENT and GLP-2 pigs had larger intestine weights, longer villi, and higher lactose digestive capacity and in vivo net glucose and galactose absorption compared with TPN alone. These endpoints were similar in ENT and GLP-2 pigs except for a lower intestinal weight and net glucose absorption in GLP-2 compared with ENT pigs. The enhanced hexose absorption in GLP-2 compared with TPN pigs corresponded with higher lactose digestive and apical glucose transport capacities, increased abundance of SGLT-1, but not GLUT-2, and lower intestinal metabolism of [ 13C]glucose to [ 13C]lactate. Our findings indicate that GLP-2 treatment during chronic TPN maintains intestinal structure and lactose digestive and hexose absorptive capacities, reduces intestinal hexose metabolism, and may facilitate the transition to enteral feeding in TPN-fed infants.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume290
Issue number2
DOIs
StatePublished - Feb 1 2006
Externally publishedYes

Fingerprint

Glucagon-Like Peptide 2
Hexoses
Total Parenteral Nutrition
Small Intestine
Glucose
Sodium-Glucose Transporter 1
Swine
Lactose
Portal Vein
Gastrointestinal Hormones
Lactase
Weights and Measures
Facilitative Glucose Transport Proteins
Large Intestine
Jugular Veins
Enteral Nutrition
Galactose
Duodenum
Carotid Arteries
Premature Infants

All Science Journal Classification (ASJC) codes

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Cite this

Glucagon-like peptide-2 protects against TPN-induced intestinal hexose malabsorption in enterally refed piglets. / Cottrell, J. J.; Stoll, B.; Buddington, Randal; Stephens, J. E.; Cui, L.; Chang, X.; Burrin, D. G.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 290, No. 2, 01.02.2006.

Research output: Contribution to journalArticle

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