Glucose biosensing using an enzyme-coated microcantilever

A. Subramanian, P. I. Oden, Stephen Kennel, K. B. Jacobson, R. J. Warmack, T. Thundat, M. J. Doktycz

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

A microcantilever-based biosensor is described. The enzyme glucose oxidase was immobilized on a micromachined silicon cantilever containing a gold coating, such as those used for atomic force microscopy. Specific, quantifiable deflection of the derivatized cantilevers was observed in the presence of the appropriate analyte. An analysis of the reaction energetics and the expected thermal response of the cantilever indicates that cantilever deflection is not simply a result of reaction-generated heat. This deflection appears to result from surface induced stresses. The combination of a highly specific enzyme and the microcantilever platform provides a unique approach for quantifying enzyme substrates without the complication of sample labeling.

Original languageEnglish (US)
Pages (from-to)385-387
Number of pages3
JournalApplied Physics Letters
Volume81
Issue number2
DOIs
StatePublished - Jul 8 2002
Externally publishedYes

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glucose
enzymes
deflection
gold coatings
oxidase
bioinstrumentation
marking
platforms
atomic force microscopy
heat
silicon

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

Cite this

Subramanian, A., Oden, P. I., Kennel, S., Jacobson, K. B., Warmack, R. J., Thundat, T., & Doktycz, M. J. (2002). Glucose biosensing using an enzyme-coated microcantilever. Applied Physics Letters, 81(2), 385-387. https://doi.org/10.1063/1.1492308

Glucose biosensing using an enzyme-coated microcantilever. / Subramanian, A.; Oden, P. I.; Kennel, Stephen; Jacobson, K. B.; Warmack, R. J.; Thundat, T.; Doktycz, M. J.

In: Applied Physics Letters, Vol. 81, No. 2, 08.07.2002, p. 385-387.

Research output: Contribution to journalArticle

Subramanian, A, Oden, PI, Kennel, S, Jacobson, KB, Warmack, RJ, Thundat, T & Doktycz, MJ 2002, 'Glucose biosensing using an enzyme-coated microcantilever', Applied Physics Letters, vol. 81, no. 2, pp. 385-387. https://doi.org/10.1063/1.1492308
Subramanian A, Oden PI, Kennel S, Jacobson KB, Warmack RJ, Thundat T et al. Glucose biosensing using an enzyme-coated microcantilever. Applied Physics Letters. 2002 Jul 8;81(2):385-387. https://doi.org/10.1063/1.1492308
Subramanian, A. ; Oden, P. I. ; Kennel, Stephen ; Jacobson, K. B. ; Warmack, R. J. ; Thundat, T. ; Doktycz, M. J. / Glucose biosensing using an enzyme-coated microcantilever. In: Applied Physics Letters. 2002 ; Vol. 81, No. 2. pp. 385-387.
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