Glutathione is involved in the early cadmium cytotoxic response in human lung carcinoma cells

Yujian Kang, M. Duane Enger

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Depletion of cellular glutathione (GSH) has been shown to sensitize A549-T27 human tumor cells to the cytotoxic effects of Cd2+. In this study the temporal and quantitative relationships between reduced cellular GSH levels and cadmium cytotoxic response in these cells were further investigated. Exposure of A549-T27 cells to 10 mM buthionine sulfoximine (BSO) for 8 h decreased their GSH level by 65%. This GSH level remained relatively constant for 8 h in the presence or absence of BSO, but recovered to 83% of the normal cellular level 24 h after removal of BSO. Exposure to 5 μM Cd2+ for 8 h did not significantly change cellular GSH levels. Pretreatment of the A549-T27 cells with 10 mM BSO for 8 h and subsequent exposure of the cells to Cd2+ for 10 days, with or without concurrent treatment of 10 mM BSO during the first 8 h of Cd2+ exposure, resulted in disappearance of the 5 μM Cd2+ threshold for cytotoxic response and reduction of the LC50 from 31 μM Cd2+ to 21 μM. Similar results were obtained when BSO pretreated cells were exposed to Cd2+ for 8 h. The threshold for cytotoxic response of 10 μM Cd2+ disappeared and the LC50 was reduced from 60 μM to 29 μM Cd2+ (with concurrent BSO treatment) and 30 μM (BSO pretreatment only). The results show that GSH plays an important role in early cellular protective responses to Cd2+.

Original languageEnglish (US)
Pages (from-to)93-101
Number of pages9
JournalToxicology
Volume48
Issue number1
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

Fingerprint

Buthionine Sulfoximine
Cadmium
Glutathione
Cells
Carcinoma
Lung
Tumors

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Glutathione is involved in the early cadmium cytotoxic response in human lung carcinoma cells. / Kang, Yujian; Duane Enger, M.

In: Toxicology, Vol. 48, No. 1, 01.01.1988, p. 93-101.

Research output: Contribution to journalArticle

@article{c260cc372f1a46da8bde30bde166261f,
title = "Glutathione is involved in the early cadmium cytotoxic response in human lung carcinoma cells",
abstract = "Depletion of cellular glutathione (GSH) has been shown to sensitize A549-T27 human tumor cells to the cytotoxic effects of Cd2+. In this study the temporal and quantitative relationships between reduced cellular GSH levels and cadmium cytotoxic response in these cells were further investigated. Exposure of A549-T27 cells to 10 mM buthionine sulfoximine (BSO) for 8 h decreased their GSH level by 65{\%}. This GSH level remained relatively constant for 8 h in the presence or absence of BSO, but recovered to 83{\%} of the normal cellular level 24 h after removal of BSO. Exposure to 5 μM Cd2+ for 8 h did not significantly change cellular GSH levels. Pretreatment of the A549-T27 cells with 10 mM BSO for 8 h and subsequent exposure of the cells to Cd2+ for 10 days, with or without concurrent treatment of 10 mM BSO during the first 8 h of Cd2+ exposure, resulted in disappearance of the 5 μM Cd2+ threshold for cytotoxic response and reduction of the LC50 from 31 μM Cd2+ to 21 μM. Similar results were obtained when BSO pretreated cells were exposed to Cd2+ for 8 h. The threshold for cytotoxic response of 10 μM Cd2+ disappeared and the LC50 was reduced from 60 μM to 29 μM Cd2+ (with concurrent BSO treatment) and 30 μM (BSO pretreatment only). The results show that GSH plays an important role in early cellular protective responses to Cd2+.",
author = "Yujian Kang and {Duane Enger}, M.",
year = "1988",
month = "1",
day = "1",
doi = "10.1016/0300-483X(88)90063-7",
language = "English (US)",
volume = "48",
pages = "93--101",
journal = "Toxicology",
issn = "0300-483X",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Glutathione is involved in the early cadmium cytotoxic response in human lung carcinoma cells

AU - Kang, Yujian

AU - Duane Enger, M.

PY - 1988/1/1

Y1 - 1988/1/1

N2 - Depletion of cellular glutathione (GSH) has been shown to sensitize A549-T27 human tumor cells to the cytotoxic effects of Cd2+. In this study the temporal and quantitative relationships between reduced cellular GSH levels and cadmium cytotoxic response in these cells were further investigated. Exposure of A549-T27 cells to 10 mM buthionine sulfoximine (BSO) for 8 h decreased their GSH level by 65%. This GSH level remained relatively constant for 8 h in the presence or absence of BSO, but recovered to 83% of the normal cellular level 24 h after removal of BSO. Exposure to 5 μM Cd2+ for 8 h did not significantly change cellular GSH levels. Pretreatment of the A549-T27 cells with 10 mM BSO for 8 h and subsequent exposure of the cells to Cd2+ for 10 days, with or without concurrent treatment of 10 mM BSO during the first 8 h of Cd2+ exposure, resulted in disappearance of the 5 μM Cd2+ threshold for cytotoxic response and reduction of the LC50 from 31 μM Cd2+ to 21 μM. Similar results were obtained when BSO pretreated cells were exposed to Cd2+ for 8 h. The threshold for cytotoxic response of 10 μM Cd2+ disappeared and the LC50 was reduced from 60 μM to 29 μM Cd2+ (with concurrent BSO treatment) and 30 μM (BSO pretreatment only). The results show that GSH plays an important role in early cellular protective responses to Cd2+.

AB - Depletion of cellular glutathione (GSH) has been shown to sensitize A549-T27 human tumor cells to the cytotoxic effects of Cd2+. In this study the temporal and quantitative relationships between reduced cellular GSH levels and cadmium cytotoxic response in these cells were further investigated. Exposure of A549-T27 cells to 10 mM buthionine sulfoximine (BSO) for 8 h decreased their GSH level by 65%. This GSH level remained relatively constant for 8 h in the presence or absence of BSO, but recovered to 83% of the normal cellular level 24 h after removal of BSO. Exposure to 5 μM Cd2+ for 8 h did not significantly change cellular GSH levels. Pretreatment of the A549-T27 cells with 10 mM BSO for 8 h and subsequent exposure of the cells to Cd2+ for 10 days, with or without concurrent treatment of 10 mM BSO during the first 8 h of Cd2+ exposure, resulted in disappearance of the 5 μM Cd2+ threshold for cytotoxic response and reduction of the LC50 from 31 μM Cd2+ to 21 μM. Similar results were obtained when BSO pretreated cells were exposed to Cd2+ for 8 h. The threshold for cytotoxic response of 10 μM Cd2+ disappeared and the LC50 was reduced from 60 μM to 29 μM Cd2+ (with concurrent BSO treatment) and 30 μM (BSO pretreatment only). The results show that GSH plays an important role in early cellular protective responses to Cd2+.

UR - http://www.scopus.com/inward/record.url?scp=0023799714&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023799714&partnerID=8YFLogxK

U2 - 10.1016/0300-483X(88)90063-7

DO - 10.1016/0300-483X(88)90063-7

M3 - Article

VL - 48

SP - 93

EP - 101

JO - Toxicology

JF - Toxicology

SN - 0300-483X

IS - 1

ER -