Glypican-2 binds to midkine

The role of glypican-2 in neuronal cell adhesion and neurite outgrowth

Nobuyuki Kurosawa, Guoyun Chen, Kenji Kadomatsu, Shinya Ikematsu, Sadatoshi Sakuma, Takashi Muramatsu

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Cell-surface heparan sulfate proteoglycans participate in molecular events that regulate cell adhesion, migration, and proliferation. The present study was performed to elucidate whether glypican-2 plays a role in interactions of neurons with midkine (MK), a heparin-binding neuroregulatory factor. MK bound to heparan sulfate chains of glypican-2 in a manner similar to syndecan-3. Microbeads coated with MK or poly-L-lysine induced clustering of glypican-2 as well as syndecan-3. Substratum-bound MK or poly-L-lysine induced cell adhesion of N2a neuroblastoma cells, while only MK promoted neurite outgrowth of these cells. Ligation of cell-surface glypican-2 with MK or an antibody against epitope-tagged glypican-2 induced cell adhesion and promoted neurite outgrowth. These results verified that cell-surface glypican-2 bound to MK and suggested that MK-glypican-2 interactions participate in neuronal cell migration and neurite outgrowth. In addition, we observed different localization of epitope-tagged glypican-2 and syndecan-3 on the surface of N2a cells; the result suggested that they may play different roles in MK-mediated neural function.

Original languageEnglish (US)
Pages (from-to)499-507
Number of pages9
JournalGlycoconjugate Journal
Volume18
Issue number6
DOIs
StatePublished - Dec 1 2001
Externally publishedYes

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Glypicans
Cell adhesion
Cell Adhesion
Syndecan-3
Lysine
Cell Movement
Epitopes
midkine
Neuronal Outgrowth
Heparan Sulfate Proteoglycans
Heparitin Sulfate
Neuroblastoma
Microspheres
Neurons
Ligation
Cluster Analysis
Heparin
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Glypican-2 binds to midkine : The role of glypican-2 in neuronal cell adhesion and neurite outgrowth. / Kurosawa, Nobuyuki; Chen, Guoyun; Kadomatsu, Kenji; Ikematsu, Shinya; Sakuma, Sadatoshi; Muramatsu, Takashi.

In: Glycoconjugate Journal, Vol. 18, No. 6, 01.12.2001, p. 499-507.

Research output: Contribution to journalArticle

Kurosawa, Nobuyuki ; Chen, Guoyun ; Kadomatsu, Kenji ; Ikematsu, Shinya ; Sakuma, Sadatoshi ; Muramatsu, Takashi. / Glypican-2 binds to midkine : The role of glypican-2 in neuronal cell adhesion and neurite outgrowth. In: Glycoconjugate Journal. 2001 ; Vol. 18, No. 6. pp. 499-507.
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