Growth of staphylococcus aureus with nafcillin in vitro induces a-toxin production and increases the lethal activity of sterile broth filtrates in a murine model

Douglas S. Kernodle, Patricia A. Mc Graw, Neil L. Barg, Barbara Menzies, Rama K.R. Voladri, Sidney Harshman

Research output: Contribution to journalArticle

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Abstract

The morbidity and mortality of Staphylococcus aureus infections remain high despite antibiotic therapy. To investigate further the observation that penicillins increase the hemolytic activity of staphylococcal cultures, 37 strains were grown in broth with and without subinhibitory nafcillin. Nafcillin stimulated hemolytic activity in nafcillin-susceptible and -resistant isolates. Sterile broth filtrates of nafcillin-associated cultures injected intraperitoneally in mice were more rapidly lethal than filtrates of the same strain grown without nafcillin. Lethality was neutralized by anti-α-toxin antisera. DNA-RNA hybridization revealed a nafcillin-associated increase in α-toxin mRNA during the postexponential growth phaseafter the activation of agr. Isolates grown in slightly inhibitory nafcillin concentrations had more α-toxin mRNA than did nafcillin-free cultures, whereas agr RNAIII levels were comparable. This suggests that nafcillin-induced α-toxin productionis not entirely attributable to agr. A supplemental regulatory mechanism may be involved.

Original languageEnglish (US)
Pages (from-to)410-419
Number of pages10
JournalJournal of Infectious Diseases
Volume172
Issue number2
DOIs
StatePublished - Jan 1 1995

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Nafcillin
Staphylococcus aureus
Growth
In Vitro Techniques
Messenger RNA
Penicillins
Immune Sera
RNA

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Growth of staphylococcus aureus with nafcillin in vitro induces a-toxin production and increases the lethal activity of sterile broth filtrates in a murine model. / Kernodle, Douglas S.; Mc Graw, Patricia A.; Barg, Neil L.; Menzies, Barbara; Voladri, Rama K.R.; Harshman, Sidney.

In: Journal of Infectious Diseases, Vol. 172, No. 2, 01.01.1995, p. 410-419.

Research output: Contribution to journalArticle

Kernodle, Douglas S. ; Mc Graw, Patricia A. ; Barg, Neil L. ; Menzies, Barbara ; Voladri, Rama K.R. ; Harshman, Sidney. / Growth of staphylococcus aureus with nafcillin in vitro induces a-toxin production and increases the lethal activity of sterile broth filtrates in a murine model. In: Journal of Infectious Diseases. 1995 ; Vol. 172, No. 2. pp. 410-419.
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