Guinea pig lymphocyte derived macrophage aggregation factor

Its separation from macrophage migration inhibitory factor

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Abstract

Lymphocytes from guinea pigs having delayed hypersensitivity to horse radish peroxidase (HRPO) when cultured in vitro with HRPO produce a large m.w. factor (>100,000 daltons) that causes peritoneal macrophages from nonimmune animals to aggregate. The macrophage aggregation factor (MAF) can be separated from macrophage migration inhibitory factor (MIF) by gel filtration of active lymphocyte supernatants on Sephadex G 150. MAF is heat stable (56°C for 30 min) but inactivated by trypsin. These data suggest that aggregation of macrophages in vitro by lymphokine rich culture supernatants is not due to MIF but is caused by a separate large m.w. factor.

Original languageEnglish (US)
JournalJournal of Immunology
Volume117
Issue number5
StatePublished - 1976
Externally publishedYes

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Macrophage Migration-Inhibitory Factors
Raphanus
Peroxidase
Guinea Pigs
Lymphocytes
Lymphokines
Delayed Hypersensitivity
Peritoneal Macrophages
Trypsin
Gel Chromatography
Hot Temperature
Macrophages
In Vitro Techniques
macrophage aggregation factor
sephadex

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

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abstract = "Lymphocytes from guinea pigs having delayed hypersensitivity to horse radish peroxidase (HRPO) when cultured in vitro with HRPO produce a large m.w. factor (>100,000 daltons) that causes peritoneal macrophages from nonimmune animals to aggregate. The macrophage aggregation factor (MAF) can be separated from macrophage migration inhibitory factor (MIF) by gel filtration of active lymphocyte supernatants on Sephadex G 150. MAF is heat stable (56°C for 30 min) but inactivated by trypsin. These data suggest that aggregation of macrophages in vitro by lymphokine rich culture supernatants is not due to MIF but is caused by a separate large m.w. factor.",
author = "Arnold Postlethwaite and Andrew Kang",
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T1 - Guinea pig lymphocyte derived macrophage aggregation factor

T2 - Its separation from macrophage migration inhibitory factor

AU - Postlethwaite, Arnold

AU - Kang, Andrew

PY - 1976

Y1 - 1976

N2 - Lymphocytes from guinea pigs having delayed hypersensitivity to horse radish peroxidase (HRPO) when cultured in vitro with HRPO produce a large m.w. factor (>100,000 daltons) that causes peritoneal macrophages from nonimmune animals to aggregate. The macrophage aggregation factor (MAF) can be separated from macrophage migration inhibitory factor (MIF) by gel filtration of active lymphocyte supernatants on Sephadex G 150. MAF is heat stable (56°C for 30 min) but inactivated by trypsin. These data suggest that aggregation of macrophages in vitro by lymphokine rich culture supernatants is not due to MIF but is caused by a separate large m.w. factor.

AB - Lymphocytes from guinea pigs having delayed hypersensitivity to horse radish peroxidase (HRPO) when cultured in vitro with HRPO produce a large m.w. factor (>100,000 daltons) that causes peritoneal macrophages from nonimmune animals to aggregate. The macrophage aggregation factor (MAF) can be separated from macrophage migration inhibitory factor (MIF) by gel filtration of active lymphocyte supernatants on Sephadex G 150. MAF is heat stable (56°C for 30 min) but inactivated by trypsin. These data suggest that aggregation of macrophages in vitro by lymphokine rich culture supernatants is not due to MIF but is caused by a separate large m.w. factor.

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