Haptoglobin release by human adipose tissue in primary culture

John N. Fain, Suleiman Bahouth, Atul K. Madan

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Haptoglobin is a putative adiposity marker because its concentration in blood is increased in obese humans. The present studies examined haptoglobin release by explants of adipose tissue in primary culture. Haptoglobin was released by explants of human visceral and subcutaneous adipose tissue at a nearly linear rate over 48 h. Explants of visceral adipose tissue released more haptoglobin than did explants of subcutaneous adipose tissue. The release of haptoglobin was quite variable, but there was a close correlation between haptoglobin release by visceral adipose tissue and that by explants of subcutaneous tissue from the same individual. Dexamethasone and niflumic acid, a cyclooxygenase-2 inhibitor, both inhibited haptoglobin release. There was release of haptoglobin by both isolated adipocytes and the adipose tissue matrix remaining after collagenase digestion of human adipose tissue. However, the amount of haptoglobin released by human adipose tissue explants in primary culture was quite low in relationship to the circulating level of haptoglobin.

Original languageEnglish (US)
Pages (from-to)536-542
Number of pages7
JournalJournal of Lipid Research
Volume45
Issue number3
DOIs
StatePublished - Mar 1 2004

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Haptoglobins
Adipose Tissue
Tissue
Intra-Abdominal Fat
Subcutaneous Fat
Niflumic Acid
human HP protein
Cyclooxygenase 2 Inhibitors
Subcutaneous Tissue
Adiposity
Collagenases
Adipocytes
Dexamethasone
Digestion
Blood

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Haptoglobin release by human adipose tissue in primary culture. / Fain, John N.; Bahouth, Suleiman; Madan, Atul K.

In: Journal of Lipid Research, Vol. 45, No. 3, 01.03.2004, p. 536-542.

Research output: Contribution to journalArticle

Fain, John N. ; Bahouth, Suleiman ; Madan, Atul K. / Haptoglobin release by human adipose tissue in primary culture. In: Journal of Lipid Research. 2004 ; Vol. 45, No. 3. pp. 536-542.
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