Heat shock protein 70 expression in overloaded skeletal muscle from young and old quail

T. R. Samelman, Stephen Alway

Research output: Contribution to journalArticle

Abstract

Stretch overload has been shown to induce the expression of a number of early response genes in skeletal muscle of Japanese quails. The inducibilty of these factors is attenuated in the muscle of old quail. Heat shock protein 70 is an early response gene and the stress of stretch overload increases its expression. The effects of aging on this expression are unknown. Therefore, the purpose of this study was to examine the expression of HSP 70 after stretch overload in muscles from young and old quail. Birds were overloaded for 24 hours and anterior latissimus dorsi (ALD) and patagialis (PAT) muscles were removed. Total RNA was isolated and HSP 70 transcripts were analyzed by northern blotting. ALD and PAT muscles showed increased HSP 70 expression in both young and old overloaded muscles compared with contralateral control. HSP 70 protein expression was greater (PAT ∼30%; ALD ∼50%) in stretch and control muscle of young birds compared to old birds. Stretch overload led to increases in HSP 70 protein of 126% and 107% in young and old PAT and 79% and 37% in young and old ALD. These results indicate that although attenuated with aging, stretch overload increases HSP 70 expression in both twitch (PAT) and tonic (ALD) skeletal muscle of Japanese quail.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998
Externally publishedYes

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Quail
HSP70 Heat-Shock Proteins
Superficial Back Muscles
Muscle
Skeletal Muscle
Muscles
Birds
Coturnix
Genes
Aging of materials
Northern Blotting
Proteins
RNA

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Heat shock protein 70 expression in overloaded skeletal muscle from young and old quail. / Samelman, T. R.; Alway, Stephen.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

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