Hematopoietically derived retinal perivascular microglia initiate uveoretinitis in experimental autoimmune uveitis

Vamsi K. Gullapalli, Jie Zhang, Geeta Pararajasegaram, Narsing A. Rao

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: Hematopoietically derived cells in the retina were studied for the expression of molecules associated with antigen presentation. Methods: Bone marrow cells of (Lewis x Brown Norway) F1 rats (LBNF1) were transplanted to sublethally irradiated Brown Norway (BN) rats to construct chimeric rats (LBNF1→BN). Each of 21 established chimeras received an adoptive transfer of uveitogenic Lewis T lymphocytes. Three rats were killed on each of 7 consecutive days. The right eye of each rat was processed for flat-mount preparation of the retina; the left eye of each was frozen for cryostat sectioning. All tissues were then stained with one of the following antibodies: OX-3 (Lewis-specific MHC class II marker), anti-ICAM, anti-B7-1, anti-TNF-α or anti-IL-1β. Results: Initial clinical signs of EAU appeared first on day 4; by day 6, full-blown EAU was noted. The flat-mount preparations revealed the presence of OX-3+ cells in the retina, perivascularly exhibiting dendritic morphology on day 2. These cells were observed in the retinal nerve fiber layer (NFL). No B7-1+, ICAM-1+, TNF-α+ or IL-1β+ cells were detected. Cryostat sections revealed positive cell staining of perivascular microglia and astrocytes in the retinal NFL with anti-IL-1β and anti-TNF-α antibodies. Conclusions: Since only perivascular bone marrow-derived cells are seen to express MHC class II molecules prior to onset of EAU, and since these cells also generate the cytokines IL-1β and TNF-α, it appears that initial presentation of antigen in the retina could be by these cells.

Original languageEnglish (US)
Pages (from-to)319-325
Number of pages7
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume238
Issue number4
StatePublished - Apr 1 2000

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Uveitis
Microglia
Interleukin-1
Retina
Antigen Presentation
Nerve Fibers
Bone Marrow Cells
Adoptive Transfer
Intercellular Adhesion Molecule-1
Astrocytes
Anti-Idiotypic Antibodies
Staining and Labeling
Cytokines
T-Lymphocytes
Antibodies

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Hematopoietically derived retinal perivascular microglia initiate uveoretinitis in experimental autoimmune uveitis. / Gullapalli, Vamsi K.; Zhang, Jie; Pararajasegaram, Geeta; Rao, Narsing A.

In: Graefe's Archive for Clinical and Experimental Ophthalmology, Vol. 238, No. 4, 01.04.2000, p. 319-325.

Research output: Contribution to journalArticle

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