Heterogeneity of a Labeled Tumor Surface Protein from a Murine Lung Carcinoma Demonstrated by Two-Dimensional Electrophoresis

Robert W. Eisinger, Stephen Kennel

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Heterogeneity of a tumor surface protein (designated TSP- 180) has been demonstrated by two-dimensional electrophoresis. Line 1 carcinoma cells derived from a spontaneous alveolar carcinoma of BALB/c mice were labeled externally with 125I by use of lactoperoxidase or metabolically with [3H]- leucine before cell proteins were solubilized with Triton X-100 detergent. Immunoprecipitates prepared with heterologous an tisera allowed comparison of two-dimensional patterns of line 1 surface proteins labeled with 125Ior 3H. The isoelectric point of 125I-labeled TSP-180 was heterogeneous and varied be tween 6.1 and 6.3. Treatment with neuraminidase shifted the pi values to between 5.9 and 6.1 and reduced, but did not eliminate, the banding heterogeneity. These data show that charge heterogeneity due to sialization, as well as other factors, exists in TSP-180.

Original languageEnglish (US)
Pages (from-to)877-881
Number of pages5
JournalCancer Research
Volume41
Issue number3
StatePublished - Mar 1 1981

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Integrin alpha6beta4
Electrophoresis
Membrane Proteins
Carcinoma
Lung
Bronchiolo-Alveolar Adenocarcinoma
Lactoperoxidase
Neoplasms
Polysorbates
Octoxynol
Isoelectric Point
Neuraminidase
Leucine
Detergents
Proteins

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Heterogeneity of a Labeled Tumor Surface Protein from a Murine Lung Carcinoma Demonstrated by Two-Dimensional Electrophoresis. / Eisinger, Robert W.; Kennel, Stephen.

In: Cancer Research, Vol. 41, No. 3, 01.03.1981, p. 877-881.

Research output: Contribution to journalArticle

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