High concentrations of p-Glu-His-Pro-NH2 (Thyrotropin-releasing hormone) occur in rat prostate

A. Eugene Pekary, Burt Sharp, Jacqueline Briggs, Harold E. Carlson, Jerome M. Hershman

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Thyrotropin-releasing hormone (TRH) immunoreactivity occurs in high concentration within the rat prostate. Previous studies have shown that the immunoreactive species consists of more than one TRH-like tripeptide which cross-reacts in the TRH radioimmunoassay. The component which was highly retained during cation exchange chromatography was subjected to a preparative scale isolation, purification and structural analysis. The methods used included methanol extraction, waterethyl ether partitioning, cation exchange chromatography, affinity chromatography, high pressure liquid chromatography, TRH radioimmunoassay, in vitro pituitary bioassay, TRH receptor assay, and amino acid analysis. The mean concentration of the predominant amino acids (Glu, His, Pro), 344 pmoles/ml, and the TRH concentration measured by TRH radioimmunoassay prior to acid hydrolysis, 372 pmoles/ml, were nearly identical. Because the material analyzed cochromatographed with synthetic TRH in several chromatographic systems, had a radioreceptor potency which was indistinguishable from that for synthetic TRH, and released TSH and prolactin but not growth hormone from rat pituitaries in vitro, it is concluded that pGlu-His-Pro-NH2 is one of the TRH-like peptides in the rat vental prostate.

Original languageEnglish (US)
Pages (from-to)915-919
Number of pages5
JournalPeptides
Volume4
Issue number6
DOIs
StatePublished - Jan 1 1983

Fingerprint

Thyrotropin-Releasing Hormone
Rats
Prostate
Radioimmunoassay
Cations
Chromatography
Thyrotropin Releasing Hormone Receptors
High pressure liquid chromatography
Affinity chromatography
pro-thyrotropin releasing hormone
Amino Acids
Bioassay
Affinity Chromatography
Structural analysis
Biological Assay
Prolactin
Ether
Growth Hormone
Purification
Methanol

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

High concentrations of p-Glu-His-Pro-NH2 (Thyrotropin-releasing hormone) occur in rat prostate. / Eugene Pekary, A.; Sharp, Burt; Briggs, Jacqueline; Carlson, Harold E.; Hershman, Jerome M.

In: Peptides, Vol. 4, No. 6, 01.01.1983, p. 915-919.

Research output: Contribution to journalArticle

Eugene Pekary, A. ; Sharp, Burt ; Briggs, Jacqueline ; Carlson, Harold E. ; Hershman, Jerome M. / High concentrations of p-Glu-His-Pro-NH2 (Thyrotropin-releasing hormone) occur in rat prostate. In: Peptides. 1983 ; Vol. 4, No. 6. pp. 915-919.
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