Vysokomolekuliarnye soevye izoingibitory tipa Baumana-Birk. Vydelenie, kharakteristika, kinetika vzaimodeǐstviia s proteinazami.

Translated title of the contribution: High molecular weight soy isoinhibitors of the Bowman-Birk type. Isolation, characteristics, and kinetics of interaction with proteinases

I. P. Gladysheva, T. Z. Sharafutdinov, N. I. Larionova

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Multiple forms of Bowman-Birk soybean inhibitor have for the first time been isolated from commercial soya flour and purified to homogeneity. Amino acid compositions and isoelectric points of the inhibitors were determined. The isolated inhibitors are shown to be related to classic (M 8000 Da, 2-II) and high molecular mass glycine-rich (M 17 000 Da, 3-II, 5-II) Bowman-Birk inhibitors. The inhibitor (2-II) was found to have two reactive sites and bind trypsin at one centre and alpha-chymotrypsin, cathepsin G and human leukocyte elastase at the other. Rate constants of the complex formation (ka) and complex dissociation (kd) were determined by following the kinetics of approaching to the steady state in a system including the enzyme, the substrate and various concentrations of the inhibitor.

Original languageRussian
Pages (from-to)281-289
Number of pages9
JournalBioorganicheskaia khimiia
Volume20
Issue number3
StatePublished - Mar 1 1994

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Cathepsin G
Leukocyte Elastase
Isoelectric Point
Flour
Soybeans
Glycine
Trypsin
Catalytic Domain
Peptide Hydrolases
Molecular Weight
Amino Acids
Enzymes
alpha-chymotrypsin

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Vysokomolekuliarnye soevye izoingibitory tipa Baumana-Birk. Vydelenie, kharakteristika, kinetika vzaimodeǐstviia s proteinazami. / Gladysheva, I. P.; Sharafutdinov, T. Z.; Larionova, N. I.

In: Bioorganicheskaia khimiia, Vol. 20, No. 3, 01.03.1994, p. 281-289.

Research output: Contribution to journalArticle

Gladysheva, I. P. ; Sharafutdinov, T. Z. ; Larionova, N. I. / Vysokomolekuliarnye soevye izoingibitory tipa Baumana-Birk. Vydelenie, kharakteristika, kinetika vzaimodeǐstviia s proteinazami. In: Bioorganicheskaia khimiia. 1994 ; Vol. 20, No. 3. pp. 281-289.
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