High-resolution magic angle spinning nuclear magnetic resonance analysis of metabolic changes in melanoma cells after induction of melanogenesis

Wei Li, Radomir Slominski, Andrzej T. Slominski

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Melanin synthesis affects melanoma behavior and tumor responsiveness to therapy; therefore, we investigated metabolic changes in melanoma cells after induction of melanogenesis. Amelanotic and melanotic melanoma cells were labeled with 13C precursors and changes in their metabolism was analyzed by high-resolution magic angle spinning (HRMAS) nuclear magnetic resonance (NMR). HRMAS NMR demonstrated clear differences in the pattern of metabolic intermediates between amelanotic and melanotic cells. Although the exact nature of the metabolites requires further investigations, our comparative studies clearly show that induction of melanogenesis is associated with changes of glucose and sodium acetate metabolism, demonstrating HRMAS NMR as a powerful and noninvasive technique to investigate such process.

Original languageEnglish (US)
Pages (from-to)282-284
Number of pages3
JournalAnalytical Biochemistry
Volume386
Issue number2
DOIs
StatePublished - Mar 15 2009

Fingerprint

Magic angle spinning
Melanoma
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Metabolism
Amelanotic Melanoma
Sodium Acetate
Melanins
Metabolites
Tumors
Glucose
Neoplasms
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

High-resolution magic angle spinning nuclear magnetic resonance analysis of metabolic changes in melanoma cells after induction of melanogenesis. / Li, Wei; Slominski, Radomir; Slominski, Andrzej T.

In: Analytical Biochemistry, Vol. 386, No. 2, 15.03.2009, p. 282-284.

Research output: Contribution to journalArticle

@article{c22d9e82c5cb4e4c8002b95832dd95ce,
title = "High-resolution magic angle spinning nuclear magnetic resonance analysis of metabolic changes in melanoma cells after induction of melanogenesis",
abstract = "Melanin synthesis affects melanoma behavior and tumor responsiveness to therapy; therefore, we investigated metabolic changes in melanoma cells after induction of melanogenesis. Amelanotic and melanotic melanoma cells were labeled with 13C precursors and changes in their metabolism was analyzed by high-resolution magic angle spinning (HRMAS) nuclear magnetic resonance (NMR). HRMAS NMR demonstrated clear differences in the pattern of metabolic intermediates between amelanotic and melanotic cells. Although the exact nature of the metabolites requires further investigations, our comparative studies clearly show that induction of melanogenesis is associated with changes of glucose and sodium acetate metabolism, demonstrating HRMAS NMR as a powerful and noninvasive technique to investigate such process.",
author = "Wei Li and Radomir Slominski and Slominski, {Andrzej T.}",
year = "2009",
month = "3",
day = "15",
doi = "10.1016/j.ab.2008.12.017",
language = "English (US)",
volume = "386",
pages = "282--284",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - High-resolution magic angle spinning nuclear magnetic resonance analysis of metabolic changes in melanoma cells after induction of melanogenesis

AU - Li, Wei

AU - Slominski, Radomir

AU - Slominski, Andrzej T.

PY - 2009/3/15

Y1 - 2009/3/15

N2 - Melanin synthesis affects melanoma behavior and tumor responsiveness to therapy; therefore, we investigated metabolic changes in melanoma cells after induction of melanogenesis. Amelanotic and melanotic melanoma cells were labeled with 13C precursors and changes in their metabolism was analyzed by high-resolution magic angle spinning (HRMAS) nuclear magnetic resonance (NMR). HRMAS NMR demonstrated clear differences in the pattern of metabolic intermediates between amelanotic and melanotic cells. Although the exact nature of the metabolites requires further investigations, our comparative studies clearly show that induction of melanogenesis is associated with changes of glucose and sodium acetate metabolism, demonstrating HRMAS NMR as a powerful and noninvasive technique to investigate such process.

AB - Melanin synthesis affects melanoma behavior and tumor responsiveness to therapy; therefore, we investigated metabolic changes in melanoma cells after induction of melanogenesis. Amelanotic and melanotic melanoma cells were labeled with 13C precursors and changes in their metabolism was analyzed by high-resolution magic angle spinning (HRMAS) nuclear magnetic resonance (NMR). HRMAS NMR demonstrated clear differences in the pattern of metabolic intermediates between amelanotic and melanotic cells. Although the exact nature of the metabolites requires further investigations, our comparative studies clearly show that induction of melanogenesis is associated with changes of glucose and sodium acetate metabolism, demonstrating HRMAS NMR as a powerful and noninvasive technique to investigate such process.

UR - http://www.scopus.com/inward/record.url?scp=59749085384&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=59749085384&partnerID=8YFLogxK

U2 - 10.1016/j.ab.2008.12.017

DO - 10.1016/j.ab.2008.12.017

M3 - Article

C2 - 19138655

AN - SCOPUS:59749085384

VL - 386

SP - 282

EP - 284

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -