Highly efficient immunoliposomes prepared with a method which is compatible with various lipid compositions

Eric Holmberg, Kazuo Maruyama, David C. Litzinger, Stephen Wright, Mark Davis, George Kabalka, Stephen Kennel, Leaf Huang

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Monoclonal antibody was conjugated to N-glutaryl-phosphatidylethanolamine in the presence of octylglucoside by using N-hydroxysulfosuccinimide as a carboxyl-activation reagent. The conjugated antibody was then incorporated into liposomes by a simple dialysis method. The method is mild and is compatible with various lipid compositions of the liposomes. We have prepared immunoliposomes containing a lung endothelium-specific monoclonal antibody and showed excellent target binding (∼75% injected dose) of the immunoliposomes in mouse. Immunoliposomes can be prepared to contain other acidic lipids such as phosphatidylserine and various amounts of cholesterol. The presence of 20% or more cholesterol in liposomes resulted in high level of target binding. We have used in these experiments a new radioactive lipid-phase marker, 111 In-DTPA-SA, which was very stable in vivo. The halflife of clearance in mouse exceeded 3 weeks.

Original languageEnglish (US)
Pages (from-to)1272-1278
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume165
Issue number3
DOIs
StatePublished - Dec 30 1989
Externally publishedYes

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Liposomes
Lipids
Chemical analysis
Cholesterol
Monoclonal Antibodies
Dialysis
Phosphatidylserines
Endothelium
Half-Life
Chemical activation
Lung
Antibodies
Experiments
phosphatidylethanolamine
octyl-beta-D-glucoside
N-hydroxysulfosuccinimide
diethylenetriaminepentaacetic acid stearylamide

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Highly efficient immunoliposomes prepared with a method which is compatible with various lipid compositions. / Holmberg, Eric; Maruyama, Kazuo; Litzinger, David C.; Wright, Stephen; Davis, Mark; Kabalka, George; Kennel, Stephen; Huang, Leaf.

In: Biochemical and Biophysical Research Communications, Vol. 165, No. 3, 30.12.1989, p. 1272-1278.

Research output: Contribution to journalArticle

Holmberg, Eric ; Maruyama, Kazuo ; Litzinger, David C. ; Wright, Stephen ; Davis, Mark ; Kabalka, George ; Kennel, Stephen ; Huang, Leaf. / Highly efficient immunoliposomes prepared with a method which is compatible with various lipid compositions. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 165, No. 3. pp. 1272-1278.
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