Histamine dilates pial arterioles of newborn pigs through prostanoid production

R. Mirro, D. W. Busija, W. M. Armstead, Charles Leffler

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

To test the hypothesis that histamine would dilate pial arterioles via a prostanoid mechanism, histamine was placed on the brain surface of anesthetized newborn pigs via a closed cranial window. Pial arteriolar diameters were measured after topical application of 10-7, 10-6, 10-5, 10-4, and 10-3 M histamine. In addition, cortical cerebrospinal fluid (CSF) was collected for radioimmunoassay of 6-ketoprostaglandin F(1α) (6-keto-PGF(1α)), thromboxane B2 (TxB2), prostaglandin D2 (PGD2), prostaglandin E2 (PGE2), and prostaglandin F(2α) (PGF(2α)). The animals were then randomized to receive either indomethacin trihydrate (5 mg/kg, iv) or an equal volume of saline (vehicle). The protocol then was repeated. Histamine (before indomethacin or vehicle infusion) caused concentration-dependent vasodilation. This vasodilation was accompanied by a significant increase in cortical CSF 6-keto-PGF(1α) and PGE2. Concentrations of TxB2, PGD2, and PGF(2α) were not altered significantly by histamine application. Pretreatment with indomethacin blocked the histamine-induced cerebral vasodilation, but vasodilation after vehicle was intact. These data imply that histamine dilates the pial arterioles of newborn pigs by stimulating the synthesis of vasodilator prostanoids.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume254
Issue number6
StatePublished - 1988

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Arterioles
Histamine
Prostaglandins
Swine
Prostaglandins F
Vasodilation
Dinoprostone
Indomethacin
Prostaglandin D2
Thromboxane B2
Cerebrospinal Fluid
Vasodilator Agents
Radioimmunoassay
Brain

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Histamine dilates pial arterioles of newborn pigs through prostanoid production. / Mirro, R.; Busija, D. W.; Armstead, W. M.; Leffler, Charles.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 254, No. 6, 1988.

Research output: Contribution to journalArticle

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