Histochemical and immunochemical localization of tyrosinase in whole tissue sections of mushrooms

Bob M. Moore, Byoung Kang, William H. Flurkey

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Vertical and cross-sectional slices of live mushrooms were blotted onto nitrocellulose. The blotted membranes were stained for tyrosinase activity using l-DOPA in the presence and absence of tyrosinase inhibitors. Histochemically stained areas of tyrosinase were present throughout the entire tissue, but the gills, stalk, and cap epidermis showed darker staining reactions. These staining reactions were eliminated if the blots were preincubated or incubated in tropolone or diethyldithiocarbamate. Immunostaining patterns of cross-sectional slices were similar to that of staining with l-DOPA.

Original languageEnglish (US)
Pages (from-to)3735-3737
Number of pages3
JournalPhytochemistry
Volume27
Issue number12
DOIs
StatePublished - 1988

Fingerprint

Monophenol Monooxygenase
Agaricales
mushrooms
Tissue
Staining and Labeling
Tropolone
tropolone
fungal anatomy
Ditiocarb
Collodion
epidermis (animal)
Epidermis
Membranes
staining
tissues

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

Cite this

Histochemical and immunochemical localization of tyrosinase in whole tissue sections of mushrooms. / Moore, Bob M.; Kang, Byoung; Flurkey, William H.

In: Phytochemistry, Vol. 27, No. 12, 1988, p. 3735-3737.

Research output: Contribution to journalArticle

Moore, Bob M. ; Kang, Byoung ; Flurkey, William H. / Histochemical and immunochemical localization of tyrosinase in whole tissue sections of mushrooms. In: Phytochemistry. 1988 ; Vol. 27, No. 12. pp. 3735-3737.
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