Histopathological and postoperative behavioral comparison of rodent oral tongue resection

Fiber-enabled CO2 laser versus electrocautery

Courtney B. Shires, Jennifer M. Saputra, Lauren King, Jerome W. Thompson, Detlef Heck, Merry E. Sebelik, John Boughter

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective. To compare operative time and hemostasis of fiber-enabled CO2 laser (FECL) energy to that of the electrocautery (EC) technique for oral tongue resection, to compare return to oral intake and preoperative weight after FECL and EC resection, and to compare histologic changes in adjacent tissue after FECL and EC resection. Study Design. Prospective animal study. Setting. Research laboratory. Subjects and Methods. The CO2 laser fiber and the Bovie cautery were each used to resect the anterior tongue in 15 adult rats. Fixative perfusion and killing were performed on postoperative day 0 (n = 10), 3 (n = 10), or 7 (n = 10). Body weight, food intake, and water intake were recorded daily for 3- and 7-day survival rats. After preparation for histologic analysis, the tongue tissue was graded with a mucosal wound-healing scale (MWHS). Results. A higher incidence of intraoperative bleeding and shorter operative times were noted in the EC group. No statistically significant difference in postoperative food or water intake between the EC and FECL groups was noted. The FECL group returned to baseline weight by postoperative day 6. MWHS scores were lower in the EC group by postoperative day 3 and lower in the FECL group by postoperative day 7. Conclusions. Both EC and FECL are effective for resection of the tongue in rats. EC has the advantage of shorter operative time and lower MWHS scores by postoperative day 3; FECL has the advantages of less intraoperative bleeding, faster return to baseline body weight, and lower MWHS score by postoperative day 7.

Original languageEnglish (US)
Pages (from-to)716-721
Number of pages6
JournalOtolaryngology - Head and Neck Surgery (United States)
Volume147
Issue number4
DOIs
StatePublished - Oct 1 2012

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Electrocoagulation
Gas Lasers
Tongue
Rodentia
Wound Healing
Operative Time
Drinking
Eating
Body Weight
Hemorrhage
Cautery
Weights and Measures
Fixatives
Hemostasis
Perfusion
Prospective Studies
Incidence

All Science Journal Classification (ASJC) codes

  • Surgery
  • Otorhinolaryngology

Cite this

Histopathological and postoperative behavioral comparison of rodent oral tongue resection : Fiber-enabled CO2 laser versus electrocautery. / Shires, Courtney B.; Saputra, Jennifer M.; King, Lauren; Thompson, Jerome W.; Heck, Detlef; Sebelik, Merry E.; Boughter, John.

In: Otolaryngology - Head and Neck Surgery (United States), Vol. 147, No. 4, 01.10.2012, p. 716-721.

Research output: Contribution to journalArticle

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