HL-60 cell growth-conditioned medium is an effective inducer of myeloperoxidase expression in K-562 human leukemia cells

Erich J. Baker, David Gerard, Elena G. Bamberger, Carmen B. Lozzio, Albert T. Ichiki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

K-562 cells were cultured in HL-60 cell growth-conditioned medium (GCM) for up to 96h. Myeloperoxidase (MPO) mRNA was transiently detected by reverse transcription-polymerase chain reaction (RT-PCR) techniques at 12, 24, and 48h. The de novo expression of MPO protein was subsequently detectable by intracellular flow cytometry at 24, 48, 72 and 96h. Immunogold staining and cytochemical analysis demonstrated granularly-sequestered MPO in approximately 40% of HL-60 GCM-cultured cells after 48h of culture. The sequential detection of MPO mRNA and MPO biosynthesis is considered an indicator of serial maturation evocative of myeloblastic cells, and suggest that K-562 cells maintain the ability to differentiate along this lineage.

Original languageEnglish (US)
Pages (from-to)1017-1025
Number of pages9
JournalLeukemia Research
Volume26
Issue number11
DOIs
StatePublished - Nov 1 2002

Fingerprint

HL-60 Cells
Conditioned Culture Medium
Peroxidase
Leukemia
Growth
Cultured Cells
Messenger RNA
Reverse Transcription
Flow Cytometry
Staining and Labeling
Polymerase Chain Reaction
Proteins

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

Cite this

HL-60 cell growth-conditioned medium is an effective inducer of myeloperoxidase expression in K-562 human leukemia cells. / Baker, Erich J.; Gerard, David; Bamberger, Elena G.; Lozzio, Carmen B.; Ichiki, Albert T.

In: Leukemia Research, Vol. 26, No. 11, 01.11.2002, p. 1017-1025.

Research output: Contribution to journalArticle

Baker, Erich J. ; Gerard, David ; Bamberger, Elena G. ; Lozzio, Carmen B. ; Ichiki, Albert T. / HL-60 cell growth-conditioned medium is an effective inducer of myeloperoxidase expression in K-562 human leukemia cells. In: Leukemia Research. 2002 ; Vol. 26, No. 11. pp. 1017-1025.
@article{4599b302b7b040d99d5f55283b72d960,
title = "HL-60 cell growth-conditioned medium is an effective inducer of myeloperoxidase expression in K-562 human leukemia cells",
abstract = "K-562 cells were cultured in HL-60 cell growth-conditioned medium (GCM) for up to 96h. Myeloperoxidase (MPO) mRNA was transiently detected by reverse transcription-polymerase chain reaction (RT-PCR) techniques at 12, 24, and 48h. The de novo expression of MPO protein was subsequently detectable by intracellular flow cytometry at 24, 48, 72 and 96h. Immunogold staining and cytochemical analysis demonstrated granularly-sequestered MPO in approximately 40{\%} of HL-60 GCM-cultured cells after 48h of culture. The sequential detection of MPO mRNA and MPO biosynthesis is considered an indicator of serial maturation evocative of myeloblastic cells, and suggest that K-562 cells maintain the ability to differentiate along this lineage.",
author = "Baker, {Erich J.} and David Gerard and Bamberger, {Elena G.} and Lozzio, {Carmen B.} and Ichiki, {Albert T.}",
year = "2002",
month = "11",
day = "1",
doi = "10.1016/S0145-2126(02)00043-7",
language = "English (US)",
volume = "26",
pages = "1017--1025",
journal = "Leukemia Research",
issn = "0145-2126",
publisher = "Elsevier Limited",
number = "11",

}

TY - JOUR

T1 - HL-60 cell growth-conditioned medium is an effective inducer of myeloperoxidase expression in K-562 human leukemia cells

AU - Baker, Erich J.

AU - Gerard, David

AU - Bamberger, Elena G.

AU - Lozzio, Carmen B.

AU - Ichiki, Albert T.

PY - 2002/11/1

Y1 - 2002/11/1

N2 - K-562 cells were cultured in HL-60 cell growth-conditioned medium (GCM) for up to 96h. Myeloperoxidase (MPO) mRNA was transiently detected by reverse transcription-polymerase chain reaction (RT-PCR) techniques at 12, 24, and 48h. The de novo expression of MPO protein was subsequently detectable by intracellular flow cytometry at 24, 48, 72 and 96h. Immunogold staining and cytochemical analysis demonstrated granularly-sequestered MPO in approximately 40% of HL-60 GCM-cultured cells after 48h of culture. The sequential detection of MPO mRNA and MPO biosynthesis is considered an indicator of serial maturation evocative of myeloblastic cells, and suggest that K-562 cells maintain the ability to differentiate along this lineage.

AB - K-562 cells were cultured in HL-60 cell growth-conditioned medium (GCM) for up to 96h. Myeloperoxidase (MPO) mRNA was transiently detected by reverse transcription-polymerase chain reaction (RT-PCR) techniques at 12, 24, and 48h. The de novo expression of MPO protein was subsequently detectable by intracellular flow cytometry at 24, 48, 72 and 96h. Immunogold staining and cytochemical analysis demonstrated granularly-sequestered MPO in approximately 40% of HL-60 GCM-cultured cells after 48h of culture. The sequential detection of MPO mRNA and MPO biosynthesis is considered an indicator of serial maturation evocative of myeloblastic cells, and suggest that K-562 cells maintain the ability to differentiate along this lineage.

UR - http://www.scopus.com/inward/record.url?scp=0036842247&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036842247&partnerID=8YFLogxK

U2 - 10.1016/S0145-2126(02)00043-7

DO - 10.1016/S0145-2126(02)00043-7

M3 - Article

VL - 26

SP - 1017

EP - 1025

JO - Leukemia Research

JF - Leukemia Research

SN - 0145-2126

IS - 11

ER -