HLA-DR alleles are associated with IDDM, but not with impaired neutrophil chemotaxis in IDDM

C. J. Gustke, Sidney Stein, T. C. Hart, W. H. Hoffman, P. J. Hanes, C. M. Russell, G. S. Schuster, S. C. Watson

Research output: Contribution to journalArticle

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Abstract

Insulin-dependent diabetes mellitus (IDDM) is a risk factor for periodontitis. Depressed neutrophil chemotaxis has been demonstrated in IDDM and in early-onset periodontitis (EOP). HLA-DR antigens are associated with both IDDM and periodontitis. This investigation sought to determine an association of HLA-DR3, -DR4, and -DR53 with impaired neutrophil chemotaxis in an IDDM sample. The neutrophil chemotaxis index of 41 diabetics and 27 controls was determined by a modified Boyden chamber method, and certain class II HLA genotypes were determined by polymerase chain-reaction amplification of genomic DNA by means of sequence-specific primers (PCR-SSP). The mean chemotaxis index of the diabetics was significantly less than that of the controls (p ≤ 0.02). HLA-DR3 (p ≤ 0.002), -DR4 (p < 0.003), and -DR53 (p ≤ 0.001) were associated with IDDM. Neutrophil chemotaxis and glucose metabolism were not significantly correlated. None of the HLA-DR alleles was associated with impaired neutrophil chemotaxis. Therefore, the neutrophil chemotaxis defect of IDDM appears to be independent of these HLA-DR-associated genes.

Original languageEnglish (US)
Pages (from-to)1497-1503
Number of pages7
JournalJournal of Dental Research
Volume77
Issue number7
DOIs
StatePublished - Jan 1 1998

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HLA-DR Antigens
Chemotaxis
Type 1 Diabetes Mellitus
Neutrophils
Alleles
HLA-DR3 Antigen
Periodontitis
HLA-DR4 Antigen
Aggressive Periodontitis
Polymerase Chain Reaction
Genotype
Glucose
Genes

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Gustke, C. J., Stein, S., Hart, T. C., Hoffman, W. H., Hanes, P. J., Russell, C. M., ... Watson, S. C. (1998). HLA-DR alleles are associated with IDDM, but not with impaired neutrophil chemotaxis in IDDM. Journal of Dental Research, 77(7), 1497-1503. https://doi.org/10.1177/00220345980770070401

HLA-DR alleles are associated with IDDM, but not with impaired neutrophil chemotaxis in IDDM. / Gustke, C. J.; Stein, Sidney; Hart, T. C.; Hoffman, W. H.; Hanes, P. J.; Russell, C. M.; Schuster, G. S.; Watson, S. C.

In: Journal of Dental Research, Vol. 77, No. 7, 01.01.1998, p. 1497-1503.

Research output: Contribution to journalArticle

Gustke, CJ, Stein, S, Hart, TC, Hoffman, WH, Hanes, PJ, Russell, CM, Schuster, GS & Watson, SC 1998, 'HLA-DR alleles are associated with IDDM, but not with impaired neutrophil chemotaxis in IDDM', Journal of Dental Research, vol. 77, no. 7, pp. 1497-1503. https://doi.org/10.1177/00220345980770070401
Gustke, C. J. ; Stein, Sidney ; Hart, T. C. ; Hoffman, W. H. ; Hanes, P. J. ; Russell, C. M. ; Schuster, G. S. ; Watson, S. C. / HLA-DR alleles are associated with IDDM, but not with impaired neutrophil chemotaxis in IDDM. In: Journal of Dental Research. 1998 ; Vol. 77, No. 7. pp. 1497-1503.
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