Host gene expression signatures discriminate between ferrets infected with genetically similar h1n1 strains

Karl Ljungberg, Alexis McBrayer, Jeremy V. Camp, Yong Kyu Chu, Ronald Tapp, Diana L. Noah, Sheila Grimes, Mary L. Proctor, Peter Liljeström, Colleen Jonsson, Carl E. Bruder

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Different respiratory viruses induce virus-specific gene expression in the host. Recent evidence, including those presented here, suggests that genetically related isolates of influenza virus induce strain-specific host gene regulation in several animal models. Here, we identified systemic strain-specific gene expression signatures in ferrets infected with pandemic influenza A/California/07/2009, A/Mexico/4482/2009 or seasonal influenza A/Brisbane/59/2007. Using uncorrelated shrunken centroid classification, we were able to accurately identify the infecting influenza strain with a combined gene expression profile of 10 selected genes, independent of the severity of disease. Another gene signature, consisting of 7 genes, could classify samples based on lung pathology. Furthermore, we identified a gene expression profile consisting of 31 probes that could classify samples based on both strain and severity of disease. Thus, we show that expression-based analysis of non-infected tissue enables distinction between genetically related influenza viruses as well as lung pathology. These results open for development of alternative tools for influenza diagnostics.

Original languageEnglish (US)
Article numbere40743
JournalPLoS ONE
Volume7
Issue number7
DOIs
StatePublished - Jul 13 2012
Externally publishedYes

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Ferrets
ferrets
Transcriptome
Gene expression
influenza
Human Influenza
Viruses
gene expression
Orthomyxoviridae
disease severity
Genes
Pathology
genes
lungs
Lung
viruses
Pandemics
pandemic
Mexico
Animal Models

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Ljungberg, K., McBrayer, A., Camp, J. V., Chu, Y. K., Tapp, R., Noah, D. L., ... Bruder, C. E. (2012). Host gene expression signatures discriminate between ferrets infected with genetically similar h1n1 strains. PLoS ONE, 7(7), [e40743]. https://doi.org/10.1371/journal.pone.0040743

Host gene expression signatures discriminate between ferrets infected with genetically similar h1n1 strains. / Ljungberg, Karl; McBrayer, Alexis; Camp, Jeremy V.; Chu, Yong Kyu; Tapp, Ronald; Noah, Diana L.; Grimes, Sheila; Proctor, Mary L.; Liljeström, Peter; Jonsson, Colleen; Bruder, Carl E.

In: PLoS ONE, Vol. 7, No. 7, e40743, 13.07.2012.

Research output: Contribution to journalArticle

Ljungberg, K, McBrayer, A, Camp, JV, Chu, YK, Tapp, R, Noah, DL, Grimes, S, Proctor, ML, Liljeström, P, Jonsson, C & Bruder, CE 2012, 'Host gene expression signatures discriminate between ferrets infected with genetically similar h1n1 strains', PLoS ONE, vol. 7, no. 7, e40743. https://doi.org/10.1371/journal.pone.0040743
Ljungberg, Karl ; McBrayer, Alexis ; Camp, Jeremy V. ; Chu, Yong Kyu ; Tapp, Ronald ; Noah, Diana L. ; Grimes, Sheila ; Proctor, Mary L. ; Liljeström, Peter ; Jonsson, Colleen ; Bruder, Carl E. / Host gene expression signatures discriminate between ferrets infected with genetically similar h1n1 strains. In: PLoS ONE. 2012 ; Vol. 7, No. 7.
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