Human alpha defensin 5 expression in the human kidney and urinary tract

Johnvid Da Spencer, David Hains, Edith Porter, Charles L. Bevins, Julianne DiRosario, Brian Becknell, Huanyu Wang, Andrew L. Schwaderer

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Background: The mechanisms that maintain sterility in the urinary tract are incompletely understood. Recent studies have implicated the importance of antimicrobial peptides (AMP) in protecting the urinary tract from infection. Here, we characterize the expression and relevance of the AMP human alpha-defensin 5 (HD5) in the human kidney and urinary tract in normal and infected subjects. Methodology/Principal Findings: Using RNA isolated from human kidney, ureter, and bladder tissue, we performed quantitative real-time PCR to show that DEFA5, the gene encoding HD5, is constitutively expressed throughout the urinary tract. With pyelonephritis, DEFA5 expression significantly increased in the kidney. Using immunoblot analysis, HD5 production also increased with pyelonephritis. Immunostaining localized HD5 to the urothelium of the bladder and ureter. In the kidney, HD5 was primarily produced in the distal nephron and collecting tubules. Using immunoblot and ELISA assays, HD5 was not routinely detected in non-infected human urine samples while mean urinary HD5 production increased with E.coli urinary tract infection. Conclusions/Significance: DEFA5 is expressed throughout the urinary tract in non-infected subjects. Specifically, HD5 is expressed throughout the urothelium of the lower urinary tract and in the collecting tubules of the kidney. With infection, HD5 expression increases in the kidney and levels become detectable in the urine. To our knowledge, our findings represent the first to quantitate HD5 expression and production in the human kidney. Moreover, this is the first report to detect the presence of HD5 in infected urine samples. Our results suggest that HD5 may have an important role in maintaining urinary tract sterility.

Original languageEnglish (US)
Article numbere31712
JournalPloS one
Volume7
Issue number2
DOIs
StatePublished - Feb 16 2012

Fingerprint

urinary tract
Urinary Tract
kidneys
Kidney
Urothelium
Pyelonephritis
Urine
Ureter
pyelonephritis
Urinary Tract Infections
ureter
Infertility
urine
human alpha-defensin 5
antimicrobial peptides
Urinary Bladder
Collecting Kidney Tubules
bladder
Peptides
Gene encoding

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Da Spencer, J., Hains, D., Porter, E., Bevins, C. L., DiRosario, J., Becknell, B., ... Schwaderer, A. L. (2012). Human alpha defensin 5 expression in the human kidney and urinary tract. PloS one, 7(2), [e31712]. https://doi.org/10.1371/journal.pone.0031712

Human alpha defensin 5 expression in the human kidney and urinary tract. / Da Spencer, Johnvid; Hains, David; Porter, Edith; Bevins, Charles L.; DiRosario, Julianne; Becknell, Brian; Wang, Huanyu; Schwaderer, Andrew L.

In: PloS one, Vol. 7, No. 2, e31712, 16.02.2012.

Research output: Contribution to journalArticle

Da Spencer, J, Hains, D, Porter, E, Bevins, CL, DiRosario, J, Becknell, B, Wang, H & Schwaderer, AL 2012, 'Human alpha defensin 5 expression in the human kidney and urinary tract', PloS one, vol. 7, no. 2, e31712. https://doi.org/10.1371/journal.pone.0031712
Da Spencer J, Hains D, Porter E, Bevins CL, DiRosario J, Becknell B et al. Human alpha defensin 5 expression in the human kidney and urinary tract. PloS one. 2012 Feb 16;7(2). e31712. https://doi.org/10.1371/journal.pone.0031712
Da Spencer, Johnvid ; Hains, David ; Porter, Edith ; Bevins, Charles L. ; DiRosario, Julianne ; Becknell, Brian ; Wang, Huanyu ; Schwaderer, Andrew L. / Human alpha defensin 5 expression in the human kidney and urinary tract. In: PloS one. 2012 ; Vol. 7, No. 2.
@article{cdc5fc8df5a4450b99b47d58be081e17,
title = "Human alpha defensin 5 expression in the human kidney and urinary tract",
abstract = "Background: The mechanisms that maintain sterility in the urinary tract are incompletely understood. Recent studies have implicated the importance of antimicrobial peptides (AMP) in protecting the urinary tract from infection. Here, we characterize the expression and relevance of the AMP human alpha-defensin 5 (HD5) in the human kidney and urinary tract in normal and infected subjects. Methodology/Principal Findings: Using RNA isolated from human kidney, ureter, and bladder tissue, we performed quantitative real-time PCR to show that DEFA5, the gene encoding HD5, is constitutively expressed throughout the urinary tract. With pyelonephritis, DEFA5 expression significantly increased in the kidney. Using immunoblot analysis, HD5 production also increased with pyelonephritis. Immunostaining localized HD5 to the urothelium of the bladder and ureter. In the kidney, HD5 was primarily produced in the distal nephron and collecting tubules. Using immunoblot and ELISA assays, HD5 was not routinely detected in non-infected human urine samples while mean urinary HD5 production increased with E.coli urinary tract infection. Conclusions/Significance: DEFA5 is expressed throughout the urinary tract in non-infected subjects. Specifically, HD5 is expressed throughout the urothelium of the lower urinary tract and in the collecting tubules of the kidney. With infection, HD5 expression increases in the kidney and levels become detectable in the urine. To our knowledge, our findings represent the first to quantitate HD5 expression and production in the human kidney. Moreover, this is the first report to detect the presence of HD5 in infected urine samples. Our results suggest that HD5 may have an important role in maintaining urinary tract sterility.",
author = "{Da Spencer}, Johnvid and David Hains and Edith Porter and Bevins, {Charles L.} and Julianne DiRosario and Brian Becknell and Huanyu Wang and Schwaderer, {Andrew L.}",
year = "2012",
month = "2",
day = "16",
doi = "10.1371/journal.pone.0031712",
language = "English (US)",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "2",

}

TY - JOUR

T1 - Human alpha defensin 5 expression in the human kidney and urinary tract

AU - Da Spencer, Johnvid

AU - Hains, David

AU - Porter, Edith

AU - Bevins, Charles L.

AU - DiRosario, Julianne

AU - Becknell, Brian

AU - Wang, Huanyu

AU - Schwaderer, Andrew L.

PY - 2012/2/16

Y1 - 2012/2/16

N2 - Background: The mechanisms that maintain sterility in the urinary tract are incompletely understood. Recent studies have implicated the importance of antimicrobial peptides (AMP) in protecting the urinary tract from infection. Here, we characterize the expression and relevance of the AMP human alpha-defensin 5 (HD5) in the human kidney and urinary tract in normal and infected subjects. Methodology/Principal Findings: Using RNA isolated from human kidney, ureter, and bladder tissue, we performed quantitative real-time PCR to show that DEFA5, the gene encoding HD5, is constitutively expressed throughout the urinary tract. With pyelonephritis, DEFA5 expression significantly increased in the kidney. Using immunoblot analysis, HD5 production also increased with pyelonephritis. Immunostaining localized HD5 to the urothelium of the bladder and ureter. In the kidney, HD5 was primarily produced in the distal nephron and collecting tubules. Using immunoblot and ELISA assays, HD5 was not routinely detected in non-infected human urine samples while mean urinary HD5 production increased with E.coli urinary tract infection. Conclusions/Significance: DEFA5 is expressed throughout the urinary tract in non-infected subjects. Specifically, HD5 is expressed throughout the urothelium of the lower urinary tract and in the collecting tubules of the kidney. With infection, HD5 expression increases in the kidney and levels become detectable in the urine. To our knowledge, our findings represent the first to quantitate HD5 expression and production in the human kidney. Moreover, this is the first report to detect the presence of HD5 in infected urine samples. Our results suggest that HD5 may have an important role in maintaining urinary tract sterility.

AB - Background: The mechanisms that maintain sterility in the urinary tract are incompletely understood. Recent studies have implicated the importance of antimicrobial peptides (AMP) in protecting the urinary tract from infection. Here, we characterize the expression and relevance of the AMP human alpha-defensin 5 (HD5) in the human kidney and urinary tract in normal and infected subjects. Methodology/Principal Findings: Using RNA isolated from human kidney, ureter, and bladder tissue, we performed quantitative real-time PCR to show that DEFA5, the gene encoding HD5, is constitutively expressed throughout the urinary tract. With pyelonephritis, DEFA5 expression significantly increased in the kidney. Using immunoblot analysis, HD5 production also increased with pyelonephritis. Immunostaining localized HD5 to the urothelium of the bladder and ureter. In the kidney, HD5 was primarily produced in the distal nephron and collecting tubules. Using immunoblot and ELISA assays, HD5 was not routinely detected in non-infected human urine samples while mean urinary HD5 production increased with E.coli urinary tract infection. Conclusions/Significance: DEFA5 is expressed throughout the urinary tract in non-infected subjects. Specifically, HD5 is expressed throughout the urothelium of the lower urinary tract and in the collecting tubules of the kidney. With infection, HD5 expression increases in the kidney and levels become detectable in the urine. To our knowledge, our findings represent the first to quantitate HD5 expression and production in the human kidney. Moreover, this is the first report to detect the presence of HD5 in infected urine samples. Our results suggest that HD5 may have an important role in maintaining urinary tract sterility.

UR - http://www.scopus.com/inward/record.url?scp=84863141810&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863141810&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0031712

DO - 10.1371/journal.pone.0031712

M3 - Article

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 2

M1 - e31712

ER -